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- 技术资料
- 抗体名:
VGLUT1 Antibody 抗体
- 抗体英文名:
VGLUT1 Antibody
- 靶点:
VGLUT1
- 浓度:
1 mg/ml
- 应用范围:
IHC, WB
- 宿主:
Mouse
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb67482
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 保存条件:
详询
- 亚型:
IgG1
- 免疫原:
详询
- 规格:
100 ug
类别: Antibodies
描述: Mouse Anti-Rat VGLUT1 Monoclonal IgG1
克隆号: N28/9 (Formerly sold as S28-9)
别名: VGLUT1, Vesicular Glutamate Transporter 1, SLC17A7, BNPI, Solute Carrier Family 17 Member 7, Brain-specific Na(+)-dependent Inorganic Phosphate Cotransporter, Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter), Member 7, Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7, VGluT1, VGLUT 1
应用稀释比例: WB (1:1000)
应用说明: 1 µg/ml was sufficient for detection of VGLut1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
免疫原: Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
分子量: 52kDa
防腐剂: PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
纯化方法: Protein G Purified
特异性: Detects ~52kDa. No cross-reactivity against VGlut2.
研究领域: Neuroscience
UniProt ID: Q62634
NCBI 参考序列: NP_446311.1
Entrez 基因 ID: 116638
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.




描述: Mouse Anti-Rat VGLUT1 Monoclonal IgG1
克隆号: N28/9 (Formerly sold as S28-9)
别名: VGLUT1, Vesicular Glutamate Transporter 1, SLC17A7, BNPI, Solute Carrier Family 17 Member 7, Brain-specific Na(+)-dependent Inorganic Phosphate Cotransporter, Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter), Member 7, Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7, VGluT1, VGLUT 1
应用稀释比例: WB (1:1000)
应用说明: 1 µg/ml was sufficient for detection of VGLut1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
免疫原: Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
分子量: 52kDa
防腐剂: PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
纯化方法: Protein G Purified
特异性: Detects ~52kDa. No cross-reactivity against VGlut2.
研究领域: Neuroscience
UniProt ID: Q62634
NCBI 参考序列: NP_446311.1
Entrez 基因 ID: 116638
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone N28/9. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT1 Antibody (D) Composite.

Immunohistochemistry analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone N28/9. Tissue: spinal cord. Species: Mouse. Fixation: 4% PFA. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:500 for 16 hours at RT. Secondary Antibody: Alexa Fluor 555 Donkey Anti-Mouse (red) at 1:2000 for 2 hours at RT. Counterstain: NeuN neuronal stain (green). Magnification: 20X.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone N28/9. Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:100. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) VGLUT1 Antibody. (D) Hoechst (E) Composite.

Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone N28/9. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:1000.
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VGLUT1 Antibody 抗体,orb67482,Biorbyt
¥6903






