- ￥8424
- Biorbyt
- orb147313
- 英国
- 2026年07月24日
- ELISA, ICC, IF, IHC, IP, WB
- Mouse
- Human, Mouse, Plant, Rat
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- 文献和实验
- 技术资料
- 抗体名：
HSP90 Antibody (Biotin) 抗体
- 抗体英文名：
HSP90 Antibody (Biotin)
- 靶点：
HSP90
- 浓度：
1 mg/ml
- 应用范围：
ELISA, ICC, IF, IHC, IP, WB
- 宿主：
Mouse
- 适应物种：
Human, Mouse, Plant, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb147313
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Biotin
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
IgG2a
- 免疫原：
详询
- 规格：
200 ug
描述: Mouse monoclonal to Hsp90 antibody (Biotin)
克隆号: 4F3.E8
别名: HSP90, HSP90AB1, HSP90-beta, HSPCB, HSPC2, Heat shock protein HSP 90-beta, Heat shock 84 kDa protein, HSP84, HSP90B
应用稀释比例: WB (1:2000), IHC (1:100), ICC/IF (1:100)
应用说明: 0.5 µg/ml was sufficient for detection of HSP90alpha in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
免疫原: Recombinant Human HSP90 purified from E.coli
分子量: 90kDa
防腐剂: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
纯化方法: Protein G Purified
特异性: Detects ~90kDa. This antibody detects both α and β forms of HSP90 equally well.
研究领域: Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction
UniProt ID: P07900
NCBI 参考序列: NP_001017963.2
Entrez 基因 ID: 3320
保存条件: Conjugated antibodies should be stored according to the product label
备注: For research use only.
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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