- ￥8424
- Biorbyt
- orb146888
- 英国
- 2026年07月24日
- AM, IHC, WB
- Mouse
- Fungi, Gallus, Human, Insect, Invertebrate, Mouse, Plant, Rabbit, Rat
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- 文献和实验
- 技术资料
- 抗体名：
HSP90 Antibody (Biotin) 抗体
- 抗体英文名：
HSP90 Antibody (Biotin)
- 靶点：
HSP90
- 浓度：
1 mg/ml
- 应用范围：
AM, IHC, WB
- 宿主：
Mouse
- 适应物种：
Fungi, Gallus, Human, Insect, Invertebrate, Mouse, Plant, Rabbit, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb146888
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Biotin
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
IgG2b
- 免疫原：
详询
- 规格：
200 ug
描述: Mouse monoclonal to Hsp90 antibody (Biotin)
克隆号: AC-16
别名: HSP90, HSP90AB1, HSP90-beta, HSPCB, HSPC2, Heat shock protein HSP 90-beta, Heat shock 84 kDa protein, HSP84, HSP90B
应用稀释比例: WB (1:1000), IHC (1:2000)
应用说明: 1 µg/ml was sufficient for detection of HSP90 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody
免疫原: Heat shock protein 90 from the water mold Achyla ambisexualis
分子量: 90kDa
防腐剂: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
纯化方法: Protein G Purified
特异性: Detects 90kDa. This antibody is reactive with both the constitutive and the inducible form of HSP90. It does not bind to the native form and does not recognize HSP90 from E.coli or yeast.
研究领域: Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction
UniProt ID: Q8LLI5
NCBI 参考序列: AAM90675.1
Entrez 基因 ID: 4768
保存条件: Conjugated antibodies should be stored according to the product label
备注: For research use only.
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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