- ￥9360
- biorbyt
- orb146803
- 英国
- 2025年12月04日
- AM, ELISA, ICC, IF, IHC, IP, WB
- Mouse
- Canine, Fish, Gallus, Hamster, Human, Mouse, Rabbit, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
HSP90 Antibody (Biotin)抗体
- 抗体英文名：
HSP90 Antibody (Biotin)
- 靶点：
HSP90
- 浓度：
1 mg/ml
- 应用范围：
AM, ELISA, ICC, IF, IHC, IP, WB
- 宿主：
Mouse
- 适应物种：
Canine, Fish, Gallus, Hamster, Human, Mouse, Rabbit, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb146803
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Biotin
- 克隆性：
Monoclonal
- 亚型：
IgG2a
- 免疫原：
Recombinant human HSP90beta
- 规格：
200 ug
别名:HSP90, HSP90AB1, HSP90-beta, HSPCB, HSPC2, Heat shock protein HSP 90-beta, Heat shock 84 kDa protein, HSP84, HSP90B
免疫原：Recombinant human HSP90beta
克隆性:H9010
分子量:90kDa
应用稀释比例：WB (1:2500), IHC (1:100)
应用注释：1 µg/ml of SMC-107 was sufficient for detection of HSP90beta in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
防腐剂:136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
纯化:Protein G Purified
保存说明:Conjugated antibodies should be stored according to the product label
NCBI:NP_031381.2
Entrez:3326
UniProt ID:P08238
Note:For research use only.
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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