辅酶Ⅰ含量检测试剂盒，用于对样本中NADH含量检测，NADH

辅酶Ⅰ含量检测试剂盒 (NADH Assay Kit)

产品概述

Micro Coenzyme Ⅰ NAD(H) Assay Kit（货号：KTB1020-C）是一款用于准确测定血清（浆）、细胞、细菌或组织提取物中NAD⁺、NADH浓度及其比值的专业试剂盒。该产品采用成熟的比色法原理，具备高灵敏度和宽线性检测范围，适用于细胞代谢及相关氧化还原反应研究。

产品详情

• 英文名称：Micro Coenzyme Ⅰ NAD(H) Assay Kit
• 中文名称：辅酶Ⅰ含量检测试剂盒
• 货号：KTB1020-C
• 检测类型：辅酶相关（NAD(H)）
• 检测方法：比色法
• 检测指标：NAD⁺、NADH
• 检测范围：0.78–50 µM（NAD⁺/NADH）

试剂盒组分

• 实验缓冲液
• EtOH溶液
• WST-8溶液
• Enhancer增强剂
• NAD酶循环反应混合物
• NAD标准品 (11 mM)
• NAD提取缓冲液
• NADH提取缓冲液

特点与优势

• 可同时检测NAD⁺与NADH浓度，并计算其比值
• 适用于多种样本类型：血清、血浆、细胞、细菌及组织提取物
• 提供宽线性范围：0.78–50 µM，满足多数实验需求
• 试剂稳定性高，重现性好

注意事项

操作时请快速加入工作试剂并充分混合，以确保反应均匀。以下物质可能干扰检测结果，建议在样本制备过程中避免：EDTA（＞0.5 mM）、抗坏血酸、SDS（＞0.2%）、NAN3、NP-40（＞1%）和吐温-20（＞1%）。

保存与运输

• 保存条件：建议避光保存于-20°C，有效期6个月
• 运输条件：冰袋（蓝冰）运输

规格与价格

规格	价格（元）	货期
48 T/48 S	898	现货（当天发货）
96 T/96 S	1469	现货（当天发货）

背景信息

烟酰胺腺嘌呤二核苷酸（NAD）是细胞中关键的氧化还原辅因子，参与多种代谢途径，包括能量代谢和信号转导。其还原形式NADH在电子传递和ATP生成中发挥核心作用。本试剂盒通过精确测定NAD⁺/NADH水平，为细胞代谢状态评估提供可靠工具。

警告

本产品仅限研究使用，不适用于人类或临床诊断。使用者应确保应用方式不违反任何专利或许可协议，本公司不对因使用本产品所导致的专利侵权或其他违规行为承担责任。

别名：NAD检测, NADH检测, NAD/NADH 检测试剂盒, NAD, NAD/NADH

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Coenzyme I Content Assay Kit (NADH Assay Kit)

Product Overview

Micro Coenzyme Ⅰ NAD(H) Assay Kit (Cat No.: KTB1020-C) is a professional kit designed for the accurate determination of NAD⁺ and NADH concentrations, as well as their ratio, in serum (plasma), cells, bacteria, or tissue extracts. Utilizing a well-established colorimetric principle, this product offers high sensitivity and a broad linear detection range, making it suitable for studies on cellular metabolism and related redox reactions.

Product Details

• English Name: Micro Coenzyme Ⅰ NAD(H) Assay Kit
• Chinese Name: Coenzyme I Content Assay Kit
• Catalog Number: KTB1020-C
• Assay Type: Coenzyme-related (NAD(H))
• Assay Method: Colorimetric
• Target Analytes: NAD⁺, NADH
• Detection Range: 0.78–50 µM (NAD⁺/NADH)

Kit Components

• Assay Buffer
• EtOH Solution
• WST-8 Solution
• Enhancer
• NAD Enzyme Cycling Reaction Mixture
• NAD Standard (11 mM)
• NAD Extraction Buffer
• NADH Extraction Buffer

Features and Advantages

• Simultaneous detection of NAD⁺ and NADH concentrations and calculation of their ratio
• Applicable to various sample types: serum, plasma, cells, bacteria, and tissue extracts
• Wide linear range: 0.78–50 µM, meeting the requirements of most experiments
• High reagent stability and excellent reproducibility

Precautions

During operation, please quickly add the working reagent and mix thoroughly to ensure uniform reaction. The following substances may interfere with the assay results and should be avoided during sample preparation: EDTA (>0.5 mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%), and Tween-20 (>1%).

Storage and Shipping

• Storage Conditions: Recommend storage protected from light at -20°C; valid for 6 months
• Shipping Conditions: Shipped with ice packs (blue ice)

Specifications and Pricing

Specification	Price (CNY)	Lead Time
48 T/48 S	898	In stock (shipped same day)
96 T/96 S	1469	In stock (shipped same day)

Background Information

Nicotinamide adenine dinucleotide (NAD) is a crucial redox cofactor in cells, involved in various metabolic pathways, including energy metabolism and signal transduction. Its reduced form, NADH, plays a central role in electron transport and ATP generation. This kit provides a reliable tool for assessing cellular metabolic status by accurately measuring NAD⁺/NADH levels.

Warning

This product is for research use only and is not intended for human or clinical diagnostic use. Users should ensure that their application does not infringe any patents or licensing agreements. The company is not liable for any patent infringements or other violations resulting from the use of this product.

Alias: NAD assay, NADH assay, NAD/NADH assay kit, NAD, NAD/NADH

Note: The above information may change due to product optimization and upgrades. For specific details, please consult Yakain Corporation.