NADH试剂盒，用于样本中辅酶Ⅰ含量检测，NADH Assa

Micro Coenzyme Ⅰ NAD(H) Assay Kit（NADH试剂盒）

货号：KTB1020-E

产品概述

本产品为细胞代谢检测试剂盒，专用于定量检测各类生物样本中辅酶Ⅰ（NAD+/NADH）的含量及其比例。适用于血清、血浆、细胞提取物、细菌及组织样本，采用高灵敏度比色法，提供稳定可靠的检测结果。

应用领域

• 细胞能量代谢研究
• 氧化还原状态评估
• 药物筛选与代谢毒理学研究
• 病原微生物代谢机制分析

试剂盒组分

• 实验缓冲液
• EtOH溶液
• WST-8溶液
• Enhancer增强剂
• NAD酶循环反应混合物
• NAD标准品 (11 mM)
• NAD提取缓冲液
• NADH提取缓冲液

主要特点与优势

• 可同时检测NAD+ 与 NADH 的浓度及比值
• 线性检测范围：0.78–50 µM，涵盖绝大多数生物样本浓度
• 操作简便，无需复杂设备，适配常规酶标仪
• 高特异性，干扰物质明确，便于实验设计规避

检测性能参数

• 检测方法：比色法（Absorbance @ 450 nm）
• 检测指标：NAD(H) 总量及 NAD+/NADH 比例
• 样本类型：细胞、细菌、组织、血清/血浆等提取物

注意事项

请避免样本中含有以下干扰物质：

• EDTA（＞0.5 mM）
• 抗坏血酸
• SDS（＞0.2%）
• NAN3
• NP-40（＞1%）
• 吐温-20（＞1%）

建议快速加入工作试剂并充分混合，以确保反应一致性。

保存与运输

• 保存条件：-20°C 避光保存，有效期6个月
• 运输方式：蓝冰冰袋运输，保障试剂稳定性

规格与价格

规格	价格（元）	货期
48 T/48 S	898	现货（当天发货）
96 T/96 S	1469	现货（当天发货）

背景介绍

烟酰胺腺嘌呤二核苷酸（NAD）是细胞能量代谢中的关键辅因子，广泛参与氧化还原反应、ADP-核糖基化及sirtuin信号通路。其氧化态（NAD+）与还原态（NADH）的比例变化常被视为细胞代谢状态的重要指标。

使用声明：本产品仅限于科学研究用途，不应用于人类或临床诊断。使用者应自行确保不侵犯任何专利或许可协议。

别名/搜索关键词：
NAD检测, NADH检测, NAD/NADH 检测试剂盒, NAD, NAD/NADH

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Micro Coenzyme Ⅰ NAD(H) Assay Kit

Catalog Number: KTB1020-E

Product Overview

This product is a cell metabolism assay kit specifically designed for the quantitative detection of Coenzyme Ⅰ (NAD+/NADH) content and its ratio in various biological samples. It is suitable for serum, plasma, cellular extracts, bacterial, and tissue samples. Utilizing a highly sensitive colorimetric method, it delivers stable and reliable detection results.

Applications

• Research on cellular energy metabolism
• Assessment of redox status
• Drug screening and metabolic toxicology studies
• Analysis of metabolic mechanisms in pathogenic microorganisms

Kit Components

• Assay Buffer
• EtOH Solution
• WST-8 Solution
• Enhancer
• NAD Enzyme Cycling Reaction Mix
• NAD Standard (11 mM)
• NAD Extraction Buffer
• NADH Extraction Buffer

Key Features & Advantages

• Simultaneous detection of NAD+ and NADH concentrations and their ratio
• Linear detection range: 0.78–50 µM, covering the concentrations in the vast majority of biological samples
• Simple operation, no complex equipment required, compatible with standard microplate readers
• High specificity, with clearly defined interfering substances to facilitate experimental design and avoidance

Assay Performance Parameters

• Detection Method: Colorimetric method (Absorbance @ 450 nm)
• Targets: Total NAD(H) and NAD+/NADH ratio
• Sample Types: Extracts from cells, bacteria, tissues, serum/plasma, etc.

Precautions

Please avoid the presence of the following interfering substances in samples:

• EDTA (>0.5 mM)
• Ascorbic Acid
• SDS (>0.2%)
• Sodium Azide
• NP-40 (>1%)
• Tween-20 (>1%)

It is recommended to add the Working Reagent quickly and mix thoroughly to ensure reaction consistency.

Storage & Shipping

• Storage Conditions: Store at -20°C protected from light; stable for 6 months
• Shipping Method: Shipped with blue ice packs to ensure reagent stability

Specifications & Pricing

Specification	Price (RMB)	Availability
48 T/48 S	898	In stock (ships same day)
96 T/96 S	1469	In stock (ships same day)

Background

Nicotinamide adenine dinucleotide (NAD) is a crucial cofactor in cellular energy metabolism, extensively involved in redox reactions, ADP-ribosylation, and sirtuin signaling pathways. The ratio of its oxidized form (NAD+) to reduced form (NADH) is often regarded as a key indicator of cellular metabolic status.

Usage Statement: This product is intended for research use only and is not for use in human or clinical diagnosis. The user is responsible for ensuring that no patents or licensing agreements are infringed.

Aliases/Search Keywords:
NAD assay, NADH assay, NAD/NADH Assay Kit, NAD, NAD/NADH

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult YaKeyin Company.