S-AI试剂盒，用于样本中可溶性酸性转化酶活性检测，S-AI

CheKine™ 可溶性酸性转化酶（S-AI）活性检测试剂盒（微量法）

货号：KTB2292-E

产品分类：核心产品 | 产品中心：细胞分析

产品概述

CheKine™ Micro Soluble Acid Anvertase (S-AI) Activity Assay Kit 是一款基于比色法原理开发的检测试剂盒，专为植物组织样本中可溶性酸性转化酶（S-AI）活性检测而优化设计。该试剂盒通过测量酶促反应产生的还原糖与显色剂反应后在510 nm处的吸光度变化，精准反映S-AI酶活性水平，适用于各类植物代谢研究场景。

背景信息

蔗糖转化酶（Invertase, Ivr）是高等植物蔗糖代谢中的关键酶，催化蔗糖分解为果糖和葡萄糖。其中，可溶性酸性转化酶（S-AI）最适pH为4.5–5.0，主要分布于植物细胞液泡或自由空间，参与调节蔗糖利用与果实糖积累过程。S-AI活性的准确测定对理解植物糖代谢机制具有重要意义。

试剂盒组分

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard

检测原理

在本试剂盒中，S-AI催化蔗糖降解生成还原糖（葡萄糖和果糖），还原糖在碱性条件下与3,5-二硝基水杨酸发生显色反应，生成棕红色化合物，在510 nm处具有特征吸收峰。在一定范围内，510 nm处吸光度随时间的变化速率与S-AI活性呈正相关。

适用样本类型

本试剂盒适用于多种植物组织样本，如果实、叶片、根茎等。

保存与运输条件

保存建议：4℃避光保存，有效期6个月。

运输条件：冰袋（蓝冰）运输。

注意事项

• 推荐使用新鲜样本进行检测。若需保存，请于-80℃环境下存放，避免反复冻融。
• 样本如在室温解冻，应在4小时内完成实验操作，以维持酶活性稳定。
• 实验前请确保所有试剂、标准品及设备处于适宜温度，以保证反应一致性。

可供选择规格与货期

规格	价格（元）	货期
48 T/24 S	598	现货（次日发货）
96 T/48 S	998	现货（次日发货）

警告：本品仅供科研使用，不适用于人类或临床诊断。使用者应自行负责确保应用不侵犯专利或许可证规定。

别名：可溶性酸性转化酶（S-AI）；S-AI Activity Assay Kit

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro Soluble Acid Invertase (S-AI) Activity Assay Kit

Catalog Number: KTB2292-E

Product Classification: Core Products | Product Center: Cell Analysis

Product Overview

The CheKine™ Micro Soluble Acid Invertase (S-AI) Activity Assay Kit is a colorimetric-based assay kit specifically optimized for the detection of soluble acid invertase (S-AI) activity in plant tissue samples. This kit accurately reflects S-AI enzyme activity levels by measuring the change in absorbance at 510 nm resulting from the reaction between enzymatically produced reducing sugars and a colorimetric reagent. It is suitable for various plant metabolism research applications.

Background Information

Invertase (Ivr) is a key enzyme in sucrose metabolism in higher plants, catalyzing the hydrolysis of sucrose into fructose and glucose. Among its isoforms, soluble acid invertase (S-AI), with an optimum pH of 4.5–5.0, is primarily located in the vacuole or free space of plant cells and is involved in regulating sucrose utilization and fruit sugar accumulation. Accurate determination of S-AI activity is crucial for understanding plant sugar metabolism mechanisms.

Kit Components

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard

Assay Principle

In this kit, S-AI catalyzes the degradation of sucrose to generate reducing sugars (glucose and fructose). Under alkaline conditions, the reducing sugars react with 3,5-dinitrosalicylic acid (DNS) to produce a brown-red colored compound, which has a characteristic absorption peak at 510 nm. Within a certain range, the rate of change in absorbance at 510 nm over time is directly proportional to S-AI activity.

Applicable Sample Types

This kit is suitable for various plant tissue samples, such as fruits, leaves, roots, and stems.

Storage and Shipping Conditions

Storage Recommendation: Store protected from light at 4°C; stable for 6 months.

Shipping Conditions: Shipped with ice packs (blue ice).

Precautions

• It is recommended to use fresh samples for assay. If storage is necessary, store at -80°C and avoid repeated freeze-thaw cycles.
• If samples are thawed at room temperature, complete the experimental procedure within 4 hours to maintain enzyme activity stability.
• Before the experiment, ensure all reagents, standards, and equipment are at appropriate temperatures to guarantee reaction consistency.

Available Specifications and Lead Time

Specification	Price (CNY)	Lead Time
48 T/24 S	598	In Stock (Ships Next Day)
96 T/48 S	998	In Stock (Ships Next Day)

Warning: This product is for research use only. It is not intended for human or clinical diagnostic use. The user is responsible for ensuring that applications do not infringe upon patent or license restrictions.

Alias: Soluble Acid Invertase (S-AI); S-AI Activity Assay Kit

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Withub Life Science.