PEPCK试剂盒，用于样本中磷酸烯醇式丙`酮酸羧激酶活性检测

PEPCK活性检测试剂盒 | 货号：KTB1126-E

本产品为磷酸烯醇式丙`酮酸羧激酶（PEPCK）活性检测试剂盒，采用优化的比色检测法，适用于多种生物样本中的PEPCK酶活性定量分析，是糖代谢与细胞能量研究中的理想工具。

产品特点与主要优势

• 广泛兼容多种样本类型：适用于动物组织、植物样本、细胞、细菌、血清（浆）及其他生物体液；
• 提供完整的检测体系：含Extraction Buffer及五种优化试剂（Reagent Ⅰ—Ⅴ），帮助用户高效完成样本制备与酶活检测；
• 操作流程清晰：配套详细说明书，涵盖样本处理步骤、实验流程与活性计算方法，降低操作差异；
• 高适配性与稳定性：适用于96孔UV微孔板，保证实验结果的一致性与可重复性。

产品信息

产品名称	货号	规格	价格（元）	货期
Micro Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay Kit	KTB1126-E	48 T/48 S	1406	现货，次日发货
Micro Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay Kit	KTB1126-E	96 T/96 S	2258	现货，次日发货

背景说明

PEPCK（磷酸烯醇式丙`酮酸羧激酶，EC 4.1.1.32）是糖异生途径中的关键酶，广泛分布于动植物、微生物及细胞中，催化草酰乙酸转化为磷酸烯醇式丙`酮酸。该酶活性直接影响葡萄糖合成与能量代谢平衡，是代谢研究中的重要指标。

试剂盒组分

• Extraction Buffer
• ReagentⅠ
• ReagentⅡ
• ReagentⅢ
• Reagent IV
• Reagent V

保存与运输条件

建议避光保存于-20°C，有效期为6个月。运输采用冰袋（蓝冰）低温保护，以确保试剂稳定性。

注意事项

• 需使用96孔UV微孔板进行检测；
• 严禁混用不同批次或不同厂家的试剂组分；
• 样本处理需在冰上操作，匀浆液应避免反复冻融，并在当日完成酶活检测；
• 实验前请确保所有试剂及设备处于适宜温度。

警告：本产品仅限研究使用，不适用于人类或临床诊断。使用者应负责确保其应用不违反相关专利或许可规定。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

PEPCK Activity Assay Kit | Cat. No.: KTB1126-E

This product is a Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay Kit, utilizing an optimized colorimetric method suitable for quantitative analysis of PEPCK enzyme activity in various biological samples. It is an ideal tool for research in glucose metabolism and cellular energy.

Features and Key Advantages

• Broad compatibility with various sample types: applicable to animal tissues, plant samples, cells, bacteria, serum (plasma), and other biological fluids;
• Comprehensive detection system: includes Extraction Buffer and five optimized reagents (Reagent I–V) to facilitate efficient sample preparation and enzyme activity detection;
• Clear operational workflow: accompanied by detailed instructions covering sample processing steps, experimental procedures, and activity calculation methods to minimize operational variability;
• High adaptability and stability: suitable for 96-well UV microplates, ensuring consistent and reproducible experimental results.

Product Information

Product Name	Cat. No.	Specification	Price (¥)	Delivery Time
Micro Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay Kit	KTB1126-E	48 T/48 S	1406	In stock, shipped next day
Micro Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay Kit	KTB1126-E	96 T/96 S	2258	In stock, shipped next day

Background Information

PEPCK (Phosphoenolpyruvate carboxykinase, EC 4.1.1.32) is a key enzyme in the gluconeogenesis pathway, widely distributed in animals, plants, microorganisms, and cells. It catalyzes the conversion of oxaloacetate to phosphoenolpyruvate. The activity of this enzyme directly influences glucose synthesis and energy metabolic balance, making it an important indicator in metabolic research.

Kit Components

• Extraction Buffer
• ReagentⅠ
• ReagentⅡ
• ReagentⅢ
• Reagent IV
• Reagent V

Storage and Shipping Conditions

It is recommended to store protected from light at -20°C. The shelf life is 6 months. Shipping is carried out with ice packs (blue ice) for low-temperature protection to ensure reagent stability.

Precautions

• Detection must be performed using 96-well UV microplates;
• Do not mix components from different batches or manufacturers;
• Sample processing should be performed on ice; homogenates should avoid repeated freeze-thaw cycles, and enzyme activity assays should be completed on the same day;
• Ensure all reagents and equipment are at appropriate temperatures before starting the experiment.

Warning: This product is for research use only. Not intended for human or clinical diagnostic use. The user is responsible for ensuring that its application does not infringe relevant patents or licensing regulations.

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult Yakeyin Company.