过氧化氢酶试剂盒，用于样本中CAT活性分析，微量法，CAT

Micro Catalase (CAT) Activity Assay Kit | 过氧化氢酶活性检测试剂盒（微量法）

产品概述

本产品为过氧化氢酶（CAT）活性分析试剂盒（微量法），货号：KTB1040-S。该试剂盒采用比色法，专门用于精准测定血清、血浆、组织或细胞裂解液以及其他生物体液样本中的过氧化氢酶活性。其设计基于CAT的过氧化功能，可有效避免其他过氧化物酶的干扰，确保检测结果的特异性和准确性。

主要特点与优势

• 高特异性：利用CAT的过氧化功能直接测定酶活，不受其他过氧化物酶影响。
• 广泛样本兼容性：适用于多种生物样本，包括血清、血浆、细胞裂解液等。
• 宽广检测范围：可检测2–75 µM浓度范围内的过氧化氢酶活性，灵敏度低至2 U/ml。
• 操作便捷：提供完整组分，优化实验流程，适合高通量筛选与研究使用。

检测原理

过氧化氢酶（EC 1.11.1.6）是一种关键抗氧化酶，广泛存在于好氧细胞中，能够催化过氧化氢（H₂O₂）分解为水和氧气，从而保护细胞免受氧化损伤。本试剂盒通过特异性反应体系，在CAT作用下使底物发生显色变化，通过比色法定量酶活性，结果稳定可靠。

试剂盒组分

• Assay Buffer (10×)
• Sample Diluent (10×)
• Lysis Buffer (10×)
• Methanol
• Formaldehyde standard (4.25 M)
• Catalase (control)
• Potassium Hydroxide
• Hydrogen Peroxide
• Chromogen
• Potassium Periodate

适用样本与应用领域

本试剂盒广泛适用于细胞代谢研究、氧化应激相关机制分析、药物筛选及疾病模型中的抗氧化能力评估，尤其适合需要精确量化CAT活性的科研场景。

保存与运输条件

产品需避光保存于-20℃。运输过程中采用冰袋（蓝冰）保障冷链。自发货日起，在推荐保存条件下有效期为6个月。

注意事项

• 如不立即使用，样本建议储存于-80℃以避免酶活性损失。
• 过热会导致CAT失活，请在样本制备与分析过程中保持低温环境。
• CAT在高稀释条件下不稳定，建议样本浓缩并在稀释后30分钟内完成测定。

规格与价格

规格	价格（元）	货期
96 T/96 S	316	现货（当天发货）
480 T/480 S	916	现货（次日发货）

警告

本产品仅用于科学研究，不适用于人类或临床诊断。使用本品应遵守相关法律法规，不得用于专利或许可证禁止的用途。对于因使用本产品可能引发的专利侵权或其他违规行为，本公司不承担任何责任。

别名：过氧化氢酶；CAT；Catalase；过氧化氢酶活性分析试剂盒

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Micro Catalase (CAT) Activity Assay Kit | Micro Catalase Activity Assay Kit

Product Overview

This product is the Catalase (CAT) Activity Assay Kit (Micro Method), Product No.: KTB1040-S. The kit employs a colorimetric method specifically designed for the accurate determination of catalase activity in serum, plasma, tissue or cell lysates, and other biological fluid samples. Its design is based on the peroxidation function of CAT, effectively avoiding interference from other peroxidases to ensure the specificity and accuracy of the detection results.

Key Features and Advantages

• High Specificity: Utilizes the peroxidation function of CAT to directly measure enzyme activity, unaffected by other peroxidases.
• Broad Sample Compatibility: Suitable for various biological samples, including serum, plasma, cell lysates, etc.
• Wide Detection Range: Can detect catalase activity within the concentration range of 2–75 µM, with sensitivity as low as 2 U/ml.
• Convenient Operation: Provides complete components and optimized experimental procedures, suitable for high-throughput screening and research applications.

Assay Principle

Catalase (EC 1.11.1.6) is a key antioxidant enzyme widely present in aerobic cells. It catalyzes the decomposition of hydrogen peroxide (H₂O₂) into water and oxygen, thereby protecting cells from oxidative damage. This kit utilizes a specific reaction system where the substrate undergoes a color change under the action of CAT, enabling quantitative measurement of enzyme activity via colorimetry, yielding stable and reliable results.

Kit Components

• Assay Buffer (10×)
• Sample Diluent (10×)
• Lysis Buffer (10×)
• Methanol
• Formaldehyde standard (4.25 M)
• Catalase (control)
• Potassium Hydroxide
• Hydrogen Peroxide
• Chromogen
• Potassium Periodate

Suitable Samples and Applications

This kit is widely applicable for research in cellular metabolism, analysis of oxidative stress-related mechanisms, drug screening, and evaluation of antioxidant capacity in disease models. It is particularly suitable for scientific research scenarios requiring precise quantification of CAT activity.

Storage and Shipping Conditions

The product must be stored protected from light at -20℃. Shipping is conducted using ice packs (blue ice) to ensure the cold chain. The validity period is 6 months from the date of shipment when stored under recommended conditions.

Precautions

• If not used immediately, samples are recommended to be stored at -80℃ to avoid loss of enzyme activity.
• Overheating can cause CAT inactivation; please maintain a low-temperature environment during sample preparation and analysis.
• CAT is unstable under high dilution conditions; it is recommended to concentrate samples and complete the assay within 30 minutes after dilution.

Specifications and Pricing

Specification	Price (¥)	Lead Time
96 T/96 S	316	In Stock (Ships Same Day)
480 T/480 S	916	In Stock (Ships Next Day)

Warning

This product is intended for research use only and is not suitable for human or clinical diagnostic use. Use of this product must comply with relevant laws and regulations and must not be used for purposes prohibited by patents or licenses. The company assumes no liability for any patent infringement or other violations that may arise from the use of this product.

Also Known As: Catalase; CAT; Catalase; Catalase Activity Assay Kit

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yacoin Company.