丙`酮酸羧化酶试剂盒，用于样本中PC活性检测，微量法，PC

丙`酮酸羧化酶（PC）活性检测试剂盒（微量法）

货号：KTB1119-S

产品分类：细胞分析核心产品

产品概述

CheKine™ 丙`酮酸羧化酶（PC）活性检测试剂盒（微量法）是一款专为研究人员设计的优质工具，用于精准检测动物组织、细胞等生物样本中丙`酮酸羧化酶的活性。本试剂盒采用成熟的比色法检测原理，操作流程清晰、结果稳定可靠，适用于多种样本类型的代谢研究。

检测原理

丙`酮酸羧化酶（PC）广泛存在于动物、霉菌和酵母的线粒体中，是糖异生途径中的关键限速酶。在该检测体系中，PC催化丙`酮酸、ATP和CO₂生成草酰乙酸，随后苹果酸脱氢酶进一步催化草酰乙酸与NADH反应生成苹果酸和NAD⁺。通过监测340 nm波长下NADH的氧化速率，即可准确计算出PC的酶活性。

组分列表（48 T/48 S）

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ

特点与优势

• 验证样本类型齐全，适用于多种动物组织与细胞样本；
• 操作流程简便，实验步骤优化，易于上手；
• 检测灵敏度高，结果重复性好。

适用样本类型

动物组织、细胞等样本。

保存条件与运输

本品建议在-20℃避光条件下保存，有效期为6个月。运输过程中采用蓝冰冰袋保护，以确保试剂性能稳定。

注意事项

• 推荐使用新鲜样本进行检测。如不能立即实验，样本可在-80℃保存数周，解冻过程需控制温度与时间，室温解冻应在4小时内完成；
• 实验前请确保所有试剂及设备处于合适温度，以保证检测准确性。

规格与价格

规格	价格（元）	货期
48 T/48 S	1358	现货，次日发货
96 T/96 S	2258	现货，次日发货

警告：本产品仅限研究使用，不适用于人类或临床诊断。使用者应自行确认是否符合相关专利或许可证规定，本公司不对因使用本产品所导致的专利侵权或其他违规行为承担责任。

别名：丙`酮酸羧化酶（PC）；Pyruvate Carboxylase Activity Assay Kit

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Pyruvate Carboxylase (PC) Activity Assay Kit (Micro Scale)

Catalog Number: KTB1119-S

Product Classification: Core Products for Cell Analysis

Overview

The CheKine™ Pyruvate Carboxylase (PC) Activity Assay Kit (Micro Scale) is a high-quality tool designed for researchers to accurately detect the activity of pyruvate carboxylase in biological samples such as animal tissues and cells. This kit employs a well-established colorimetric detection principle, featuring a clear procedure and reliable results, making it suitable for metabolic studies involving various sample types.

Assay Principle

Pyruvate carboxylase (PC), widely present in the mitochondria of animals, molds, and yeasts, is a key rate-limiting enzyme in the gluconeogenesis pathway. In this assay system, PC catalyzes the conversion of pyruvate, ATP, and CO₂ into oxaloacetate. Subsequently, malate dehydrogenase further catalyzes the reaction of oxaloacetate with NADH to produce malate and NAD⁺. By monitoring the oxidation rate of NADH at 340 nm, the enzymatic activity of PC can be accurately calculated.

Component List (48 T/48 S)

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ

Features and Advantages

• Comprehensive validation of sample types, suitable for various animal tissues and cell samples;
• Simple and optimized operational procedure, easy to use;
• High detection sensitivity and excellent result reproducibility.

Applicable Sample Types

Animal tissues, cells, and other samples.

Storage Conditions and Shipping

It is recommended to store this product protected from light at -20°C, with a shelf life of 6 months. During transportation, blue ice packs are used to ensure the stability of the reagents.

Precautions

• Fresh samples are recommended for detection. If immediate testing is not possible, samples can be stored at -80°C for several weeks. The thawing process should control temperature and time; thawing at room temperature should be completed within 4 hours;
• Before the experiment, ensure all reagents and equipment are at appropriate temperatures to guarantee assay accuracy.

Specifications and Pricing

Specification	Price (¥)	Lead Time
48 T/48 S	1358	In stock, shipped the next day
96 T/96 S	2258	In stock, shipped the next day

Warning: This product is for research use only. It is not intended for human or clinical diagnostic use. Users should independently confirm compliance with relevant patents or licensing regulations. The company is not liable for patent infringements or other violations resulting from the use of this product.

Alias: Pyruvate Carboxylase (PC); Pyruvate Carboxylase Activity Assay Kit

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult ArKayBiotech.