S-NPT试剂盒，用于样本中土壤中性蛋白酶活性检测，S-NP

产品概述

KTB4034-E S-NPT Activity Assay Kit是一款专为土壤样本中中性蛋白酶（S-NPT）活性检测所设计的高灵敏度试剂盒。该产品隶属于CheKine™细胞代谢检测系列，适用于环境科学研究、农业土壤肥力评估及生态监测中的酶活性分析。

检测原理

土壤中性蛋白酶（S-NPT）在近中性条件下催化蛋白质水解，生成可检测产物。本试剂盒通过酶促反应产生酪氨酸，并在碱性条件下与特定试剂发生显色反应，在680 nm波长处测定吸光度，从而准确计算S-NPT活性。

组分列表

• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Reagent V
• Reagent VI
• Standard

特点与优势

• 经验证适用于多种土壤类型，兼容性广泛
• 操作流程优化，实验步骤简便，重复性高
• 提供标准品，便于定量和结果校准

样本处理建议

推荐使用新鲜土壤样本。如需保存，请置于-80℃环境，避免反复冻融。测定前应在室温下缓慢解冻，并于4小时内完成实验，以保持酶活性稳定。

保存与运输

试剂盒需在4℃避光条件下保存，有效期6个月。运输全程采用蓝冰冰袋保温，保证试剂稳定性。

规格与定价

规格	价格（元）	货期
48 T/48 S	598	现货（次日发货）
96 T/96 S	1098	现货（次日发货）

注意事项

实验前请确保所有组分恢复至适宜温度，避免反复冻融试剂。本产品仅用于科学研究，不适用于人类或临床诊断。

背景信息

土壤中性蛋白酶（S-NPT）是土壤氮循环过程中的关键酶之一，参与降解蛋白质类物质为植物可吸收的氮源。其活性与土壤理化性质、有机质含量及微生物活动密切相关，是评估土壤健康与肥力的重要指标。

别名：土壤中性蛋白酶（S-NPT）

检测指标：土壤中性蛋白酶（S-NPT）活性

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

The KTB4034-E S-NPT Activity Assay Kit is a highly sensitive kit specifically designed for the detection of neutral protease (S-NPT) activity in soil samples. This product belongs to the CheKine™ Cell Metabolism Assay series and is suitable for enzymatic activity analysis in environmental science research, agricultural soil fertility assessment, and ecological monitoring.

Assay Principle

Soil neutral protease (S-NPT) catalyzes the hydrolysis of proteins under near-neutral conditions, generating detectable products. This kit utilizes an enzymatic reaction to produce tyrosine, which undergoes a colorimetric reaction with specific reagents under alkaline conditions. The absorbance is measured at 680 nm to accurately calculate S-NPT activity.

Components List

• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Reagent V
• Reagent VI
• Standard

Features and Advantages

• Validated for various soil types with broad compatibility
• Optimized protocol with simplified procedures and high reproducibility
• Includes standard for quantification and result calibration

Sample Handling Recommendations

Fresh soil samples are recommended. If storage is necessary, samples should be kept at -80°C and avoided repeated freeze-thaw cycles. Prior to assay, thaw slowly at room temperature and complete the experiment within 4 hours to maintain enzyme activity stability.

Storage and Shipping

The kit should be stored protected from light at 4°C and is stable for 6 months. Shipping is performed with blue ice packs to ensure reagent stability throughout transit.

Specifications and Pricing

Specification	Price (¥)	Availability
48 T/48 S	598	In stock (ships next day)
96 T/96 S	1098	In stock (ships next day)

Precautions

Ensure all components are equilibrated to appropriate temperature before use. Avoid repeated freezing and thawing of reagents. This product is for research use only, not for human or clinical diagnostic use.

Background Information

Soil neutral protease (S-NPT) is a key enzyme in the soil nitrogen cycle, involved in degrading proteinaceous materials into plant-absorbable nitrogen sources. Its activity is closely related to soil physicochemical properties, organic matter content, and microbial activity, making it an important indicator for assessing soil health and fertility.

Alias: Soil Neutral Protease (S-NPT)

Detection Target: Soil Neutral Protease (S-NPT) Activity

Note: The above information is subject to change due to product optimization and upgrades. For detailed information, please consult Yakeyin Company.