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GNPTAB Rabbit pAb, HRP conjuga

ted(bs-13476R-HRP)-100ul
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  • ￥2980
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  • bs-13476R-HRP
  • 2025年12月01日
  • 产品信息以Bioss网站为准
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    • 规格：

      100ul

    产品编号bs-13476R-HRP
    英文名称GNPTAB Rabbit pAb, HRP conjugated
    中文名称HRP标记的溶酶体累积病相关蛋白/口吃相关蛋白抗体
    英文别名N-acetylglucosamine-1-phosphotransferase subunit beta; EC=2.7.8.17; GlcNAc-1-phosphotransferase subunits alpha/beta; GNPTA; GNPTA_HUMAN; Gnptab; KIAA1208; Stealth protein GNPTAB; UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta.
    产品应用WB=1000-10000, IHC-P=1:100-500, IHC-F=1:100-500, ELISA=1:500-5000

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human N-acetylglucosamine-1-phosphotransferase subunit beta
    亚型IgG
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    亚基Hexamer of two alpha, two beta and two gamma subunits; disulfide-linked. It is believed that the alpha and/or the beta subunit of the enzyme contain the catalytic portion and that the gamma subunit functions in recognition of the lysosomal enzymes.
    亚细胞定位N-acetylglucosamine-1-phosphotransferase subunit alpha: Golgi apparatus membrane; Single-pass type I membrane protein.
    N-acetylglucosamine-1-phosphotransferase subunit beta: Golgi apparatus membrane; Single-pass type II membrane protein.
    组织特异性Expressed in the heart, whole brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    翻译后修饰The alpha- and beta-subunits appear to be generated by a proteolytic cleavage at the Lys-928-Asp-929 bond.
    相似性Belongs to the stealth family.
    Contains 1 EF-hand domain.
    Contains 2 LNR (Lin/Notch) repeats.
    功能Catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    背景资料This gene encodes two of three subunit types of the membrane-bound enzyme N-acetylglucosamine-1-phosphotransferase, a heterohexameric complex composed of two alpha, two beta, and two gamma subunits. The encoded protein is proteolytically cleaved at the Lys928-Asp929 bond to yield mature alpha and beta polypeptides while the gamma subunits are the product of a distinct gene (GeneID 84572). In the Golgi apparatus, the heterohexameric complex catalyzes the first step in the synthesis of mannose 6-phosphate recognition markers on certain oligosaccharides of newly synthesized lysosomal enzymes. These recognition markers are essential for appropriate trafficking of lysosomal enzymes. Mutations in this gene have been associated with both mucolipidosis II and mucolipidosis IIIA.[provided by RefSeq, May 2010].

     

    应用推荐稀释比例
    {WB}{1000-10000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {ELISA}{1:500-5000}

     

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    图标文献和实验
    相关实验

    • 【交流】谁用过RD公司的HRP酶标羊抗鼠IgG试剂盒.

      affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C

    • Western Immunoblotting of Proteins

      . For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes

    • Western Immunoblotting of Proteins

      antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water

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