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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
pACYC184
- 保质期:
1年
- 供应商:
再康生物
- 保存条件:
低温运输,-80℃保存
质粒编号: 60908-370y
质粒名称: pACYC184
其他名称:
质粒大小(bp): 4245
抗性:Chloramphenicol
质粒类型:原核表达系统载体
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:低拷贝
蛋白标签:
测序引物:
GenBank号: X06403
其他属性: The sequence is circular; however, the sequence begins with a unique EcoRI site. Bases 426-706, 1153-1583, and 2918 to 3735 were determined while the rest of the sequence was taken from the published sequence of pSC101, Tn9, and p15A. Restriction digests of the clone give the following sizes (kb): EcoRI–4.3; EcoRI/BamHI–2.4, 1.9. (ATCC staff) Medium is 1675 LB plus chloramphenicol (10 ug/ml). Hosts: E.coli PM191, E.coli C600 chi1776, E.coli. Related vectors: Tn9, pSC101, p15A.
质粒图谱:
质粒序列:
| 1 | gaattccgga | tgagcattca | tcaggcgggc | aagaatgtga | ataaaggccg | 50 |
| 51 | gataaaactt | gtgcttattt | ttctttacgg | tctttaaaaa | ggccgtaata | 100 |
| 101 | tccagctgaa | cggtctggtt | ataggtacat | tgagcaactg | actgaaatgc | 150 |
| 151 | ctcaaaatgt | tctttacgat | gccattggga | tatatcaacg | gtggtatatc | 200 |
| 201 | cagtgatttt | tttctccatt | ttagcttcct | tagctcctga | aaatctcgat | 250 |
| 251 | aactcaaaaa | atacgcccgg | tagtgatctt | atttcattat | ggtgaaagtt | 300 |
| 301 | ggaacctctt | acgtgccgat | caacgtctca | ttttcgccaa | aagttggccc | 350 |
| 351 | agggcttccc | ggtatcaaca | gggacaccag | gatttattta | ttctgcgaag | 400 |
| 401 | tgatcttccg | tcacaggtat | ttattcggcg | caaagtgcgt | cgggtgatgc | 450 |
| 451 | tgccaactta | ctgatttagt | gtatgatggt | gtttttgagg | tgctccagtg | 500 |
| 501 | gcttctgttt | ctatcagctg | tccctcctgt | tcagctactg | acggggtggt | 550 |
| 551 | gcgtaacggc | aaaagcaccg | ccggacatca | gcgctagcgg | agtgtatact | 600 |
| 601 | ggcttactat | gttggcactg | atgagggtgt | cagtgaagtg | cttcatgtgg | 650 |
| 651 | caggagaaaa | aaggctgcac | cggtgcgtca | gcagaatatg | tgatacagga | 700 |
| 701 | tatattccgc | ttcctcgctc | actgactcgc | tacgctcggt | cgttcgactg | 750 |
| 751 | cggcgagcgg | aaatggctta | cgaacggggc | ggagatttcc | tggaagatgc | 800 |
| 801 | caggaagata | cttaacaggg | aagtgagagg | gccgcggcaa | agccgttttt | 850 |
| 851 | ccataggctc | cgcccccctg | acaagcatca | cgaaatctga | cgctcaaatc | 900 |
| 901 | agtggtggcg | aaacccgaca | ggactataaa | gataccaggc | gtttccccct | 950 |
| 951 | ggcggctccc | tcgtgcgctc | tcctgttcct | gcctttcggt | ttaccggtgt | 1000 |
| 1001 | cattccgctg | ttatggccgc | gtttgtctca | ttccacgcct | gacactcagt | 1050 |
| 1051 | tccgggtagg | cagttcgctc | caagctggac | tgtatgcacg | aaccccccgt | 1100 |
| 1101 | tcagtccgac | cgctgcgcct | tatccggtaa | ctatcgtctt | gagtccaacc | 1150 |
| 1151 | cggaaagaca | tgcaaaagca | ccactggcag | cagccactgg | taattgattt | 1200 |
| 1201 | agaggagtta | gtcttgaagt | catgcgccgg | ttaaggctaa | actgaaagga | 1250 |
| 1251 | caagttttgg | tgactgcgct | cctccaagcc | agttacctcg | gttcaaagag | 1300 |
| 1301 | ttggtagctc | agagaacctt | cgaaaaaccg | ccctgcaagg | cggttttttc | 1350 |
| 1351 | gttttcagag | caagagatta | cgcgcagacc | aaaacgatct | caagaagatc | 1400 |
| 1401 | atcttattaa | tcagataaaa | tatttctaga | tttcagtgca | atttatctct | 1450 |
| 1451 | tcaaatgtag | cacctgaagt | cagccccata | cgatataagt | tgtaattctc | 1500 |
| 1501 | atgtttgaca | gcttatcatc | gataagcttt | aatgcggtag | tttatcacag | 1550 |
| 1551 | ttaaattgct | aacgcagtca | ggcaccgtgt | atgaaatcta | acaatgcgct | 1600 |
| 1601 | catcgtcatc | ctcggcaccg | tcaccctgga | tgctgtaggc | ataggcttgg | 1650 |
| 1651 | ttatgccggt | actgccgggc | ctcttgcggg | atatcgtcca | ttccgacagc | 1700 |
| 1701 | atcgccagtc | actatggcgt | gctgctagcg | ctatatgcgt | tgatgcaatt | 1750 |
| 1751 | tctatgcgca | cccgttctcg | gagcactgtc | cgaccgcttt | ggccgccgcc | 1800 |
| 1801 | cagtcctgct | cgcttcgcta | cttggagcca | ctatcgacta | cgcgatcatg | 1850 |
| 1851 | gcgaccacac | ccgtcctgtg | gatcctctac | gccggacgca | tcgtggccgg | 1900 |
| 1901 | catcaccggc | gccacaggtg | cggttgctgg | cgcctatatc | gccgacatca | 1950 |
| 1951 | ccgatgggga | agatcgggct | cgccacttcg | ggctcatgag | cgcttgtttc | 2000 |
| 2001 | ggcgtgggta | tggtggcagg | ccccgtggcc | gggggactgt | tgggcgccat | 2050 |
| 2051 | ctccttgcat | gcaccattcc | ttgcggcggc | ggtgctcaac | ggcctcaacc | 2100 |
| 2101 | tactactggg | ctgcttccta | atgcaggagt | cgcataaggg | agagcgtcga | 2150 |
| 2151 | ccgatgccct | tgagagcctt | caacccagtc | agctccttcc | ggtgggcgcg | 2200 |
| 2201 | gggcatgact | atcgtcgccg | cacttatgac | tgtcttcttt | atcatgcaac | 2250 |
| 2251 | tcgtaggaca | ggtgccggca | gcgctctggg | tcattttcgg | cgaggaccgc | 2300 |
| 2301 | tttcgctgga | gcgcgacgat | gatcggcctg | tcgcttgcgg | tattcggaat | 2350 |
| 2351 | cttgcacgcc | ctcgctcaag | ccttcgtcac | tggtcccgcc | accaaacgtt | 2400 |
| 2401 | tcggcgagaa | gcaggccatt | atcgccggca | tggcggccga | cgcgctgggc | 2450 |
| 2451 | tacgtcttgc | tggcgttcgc | gacgcgaggc | tggatggcct | tccccattat | 2500 |
| 2501 | gattcttctc | gcttccggcg | gcatcgggat | gcccgcgttg | caggccatgc | 2550 |
| 2551 | tgtccaggca | ggtagatgac | gaccatcagg | gacagcttca | aggatcgctc | 2600 |
| 2601 | gcggctctta | ccagcctaac | ttcgatcact | ggaccgctga | tcgtcacggc | 2650 |
| 2651 | gatttatgcc | gcctcggcga | gcacatggaa | cgggttggca | tggattgtag | 2700 |
| 2701 | gcgccgccct | ataccttgtc | tgcctccccg | cgttgcgtcg | cggtgcatgg | 2750 |
| 2751 | agccgggcca | cctcgacctg | aatggaagcc | ggcggcacct | cgctaacgga | 2800 |
| 2801 | ttcaccactc | caagaattgg | agccaatcaa | ttcttgcgga | gaactgtgaa | 2850 |
| 2851 | tgcgcaaacc | aacccttggc | agaacatatc | catcgcgtcc | gccatctcca | 2900 |
| 2901 | gcagccgcac | gcggcgcatc | tcgggcagcg | ttgggtcctg | gccacgggtg | 2950 |
| 2951 | cgcatgatcg | tgctcctgtc | gttgaggacc | cggctaggct | ggcggggttg | 3000 |
| 3001 | ccttactggt | tagcagaatg | aatcaccgat | acgcgagcga | acgtgaagcg | 3050 |
| 3051 | actgctgctg | caaaacgtct | gcgacctgag | caacaacatg | aatggtcttc | 3100 |
| 3101 | ggtttccgtg | tttcgtaaag | tctggaaacg | cggaagtccc | ctacgtgctg | 3150 |
| 3151 | ctgaagttgc | ccgcaacaga | gagtggaacc | aaccggtgat | accacgatac | 3200 |
| 3201 | tatgactgag | agtcaacgcc | atgagcggcc | tcatttctta | ttctgagtta | 3250 |
| 3251 | caacagtccg | caccgctgtc | cggtagctcc | ttccggtggg | cgcggggcat | 3300 |
| 3301 | gactatcgtc | gccgcactta | tgactgtctt | ctttatcatg | caactcgtag | 3350 |
| 3351 | gacaggtgcc | ggcagcgccc | aacagtcccc | cggccacggg | gcctgccacc | 3400 |
| 3401 | atacccacgc | cgaaacaagc | gccctgcacc | attatgttcc | ggatctgcat | 3450 |
| 3451 | cgcaggatgc | tgctggctac | cctgtggaac | acctacatct | gtattaacga | 3500 |
| 3501 | agcgctaacc | gtttttatca | ggctctggga | ggcagaataa | atgatcatat | 3550 |
| 3551 | cgtcaattat | tacctccacg | gggagagcct | gagcaaactg | gcctcaggca | 3600 |
| 3601 | tttgagaagc | acacggtcac | actgcttccg | gtagtcaata | aaccggtaaa | 3650 |
| 3651 | ccagcaatag | acataagcgg | ctatttaacg | accctgccct | gaaccgacga | 3700 |
| 3701 | ccgggtcgaa | tttgctttcg | aatttctgcc | attcatccgc | ttattatcac | 3750 |
| 3751 | ttattcaggc | gtagcaccag | gcgtttaagg | gcaccaataa | ctgccttaaa | 3800 |
| 3801 | aaaattacgc | cccgccctgc | cactcatcgc | agtactgttg | taattcatta | 3850 |
| 3851 | agcattctgc | cgacatggaa | gccatcacag | acggcatgat | gaacctgaat | 3900 |
| 3901 | cgccagcggc | atcagcacct | tgtcgccttg | cgtataatat | ttgcccatgg | 3950 |
| 3951 | tgaaaacggg | ggcgaagaag | ttgtccatat | tggccacgtt | taaatcaaaa | 4000 |
| 4001 | ctggtgaaac | tcacccaggg | attggctgag | acgaaaaaca | tattctcaat | 4050 |
| 4051 | aaacccttta | gggaaatagg | ccaggttttc | accgtaacac | gccacatctt | 4100 |
| 4101 | gcgaatatat | gtgtagaaac | tgccggaaat | cgtcgtggta | ttcactccag | 4150 |
| 4151 | agcgatgaaa | acgtttcagt | ttgctcatgg | aaaacggtgt | aacaagggtg | 4200 |
| 4201 | aacactatcc | catatcacca | gctcaccgtc | tttcattgcc | atacg | 4245 |
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文献和实验【求助】请教一下各位大虾:质粒pACYC184和pBRM-Wt的具体结构是怎样的呢,从哪可以得到,谢谢!
西点6688 请教一下各位大虾:质粒pACYC184和pBRM-Wt的具体结构是怎样的呢,谢谢! slytjiaofei http://genome-www.stanford.edu/vectordb/vector.html 自己查查吧!!! lastone9853 这两个质粒pACYC184和pBRM-Wt的图谱,序列等可以在网上查到,我这有这两个载体,可以快
系列):高拷贝会导致大片段质粒复制压力大,易丢失或断裂,优先用低拷贝载体(如 pACYC184,拷贝数 10-15)或大片段专用载体(如 BAC 载体 pBeloBAC11,可容纳 100kb 以上片段,在大肠杆菌中稳定存在); ④选择合适的连接方法,比如 Gibson 组装(无缝克隆)的方法更适合 10-20kb 大片段的连接,相比传统的 T4 连接的方法,成功率更高,操作更简便; ⑤选择重组缺陷型感受态,提高大质粒的转化效率,如 Stbl3、DH10B 等,此外转化方式也会影响转化
Strain RDP146 (F- recA' dlac-pro) rpsE ; spectinomycin resistant) with plasmid pLB101 (pACYC184 with tnpA ; active transposase, chloramphenicol resistant)(F. Heffron) #111/B428 Strain RDP146 with pOX38 F factor derivative
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