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- 文献和实验
- 技术资料
- 库存：
1
- 英文名：
EpiQuik General Protein-DNA Binding Assay Kit (Colorimetric)
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- 通用蛋白-DNA结合分析试剂盒(Colorimetric)详细信息：
-
Product Overview
The EpiQuik™ General Protein-DNA Binding Assay Kit (Colorimetric) (96 assays) is a convenient package of tools that allows the experimenter to investigate protein-DNA interaction in vitro efficiently. The entire procedure can be completed within 3 hours and produces far superior results than any competitor kits. The EpiQuik™ General Protein-DNA Binding Assay Kits are suitable for analyzing protein-DNA interactions in vitro, specifically for detecting transcription factor activation using mammalian tissue and cell extracts.
WHY CHOOSE THE EPIQUIK™ GENERAL PROTEIN-DNA BINDING ASSAY KIT (COLORIMETRIC)(96 ASSAYS)?
- The fastest procedure available: a complete assay can be finished within 3 hours.
- Strip microplate format makes the assay flexible: manual or high throughput analysis.
- Colorimetrically quantifies protein activation and uses radioactive-free materials: safer to handle.
- Simple, reliable, and consistent assay conditions.
通用蛋白-DNA结合分析试剂盒(Colorimetric)Principle & Procedure
The EpiQuik™ General Protein-DNA Binding Assay Kit (Colorimetric) is designed for measuring the transcription factor of DNA binding activity in nuclear extracts. In an assay with the use of this kit, a biotin-labeled double stranded oligonucleotide (oligo) containing DNA binding consensus sequence for the target transcription factor is incubated with nuclear extract in the binding assay buffer. Active form of transcription factor in the nuclear extract binds to its consensus sequence. Oligo-protein complex is then captured onto the assay microwell. The target protein can be recognized by high affinity antibody and colorimetrically measured through detection antibody-color development reagent reaction system.
SCHEMATIC PROCEDURE: 通用蛋白-DNA结合分析试剂盒(Colorimetric)Product Components
PD1 (10x wash buffer)
PD2 (assay binding buffer)
PD3 (antibody dilution buffer)
PD4 (developing solution)
PD5 (stop solution)
8-well assay strips (with frame)
User guide
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Colorimetric Assays for Screening Laccases
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