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- 英文名：
EpiQuik Global Pan-Methyl Histone H3-K9 Quantification Kit (Colorimetric)
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- 通用Pan-Methyl组蛋白H3-K9定量试剂盒(Colorimetric) 详细信息：
-
Product Overview
The EpiQuik™ Global Pan-Methyl Histone H3-K9 Quantification Kit (Colorimetric) is a convenient package of tools that allows the experimenter to specifically measure global mono-, di- and tri-methylation of histone H3-K9 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
WHY CHOOSE THE EPIQUIK™ GLOBAL PAN-METHYL HISTONE H3-K9 QUANTIFICATION KIT (COLORIMETRIC)?
- Quick and efficient, which can be finished within 2.5 hours.
- Innovative colorimetric assay with no need for radioactivity, electrophoresis, and chromatography.
- Simultaneous quantification of mono-, di-, and tri-methylated H3-K9 with the detection limit as low as 1 ng/well and detection range from 20 ng-5 µg/well of histone extracts.
- The control is conveniently included for quantification of the amount of mono-, di– and tri-methylated H3-K9.
- Strip microplate format makes the assay flexible: manual or high throughput.
- Simple, reliable, and consistent assay conditions.
通用Pan-Methyl组蛋白H3-K9定量试剂盒(Colorimetric) Principle & Procedure
The EpiQuik™ Global Pan-Methyl Histone H3-K9 Quantification Kit (Colorimetric) is designed for simultaneously measuring global histone H3-K9 mono-, di-, and tri-methylation. In an assay with this kit, the methylated histone H3 at lysine 9 is captured to the strip wells coated with antibodies specifically for mono-, di-, and tri-methyl H3-K9. The captured mono-, di– and tri-methylated histone H3-K9 can be then detected with a labeled detection antibody followed by color development reagent. The ratio of mono-, di-, and tri-methylated H3-K9 is proportional to the intensity of absorbance. The absolute amount of the methylated H3-K9 can be quantitated by comparing to the standard control.
SCHEMATIC PROCEDURE: 通用Pan-Methyl组蛋白H3-K9定量试剂盒(Colorimetric) Product Components
C1 (10X lysis buffer)
C2 (antibody buffer)
C3 (detection antibody, 1 mg/ml)*
C4 (color developer)
C5 (stop solution)
Standard control (100 µg/ml)*
8 well sample strips (with frame)#
8 well standard control strips
User guide
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zkB104Hu 辅肌动蛋白α3(ACTN3)ELISA试剂盒 检测范围
zkB105Hu 肌球蛋白轻链1(MYL1)ELISA试剂盒 检测范围
zkB106Hu 肌球蛋白轻链激酶(MYLK)ELISA试剂盒 检测范围
zkA086Hu L选择素(SELL)ELISA试剂盒 检测范围
zkA087Hu 单核细胞趋化蛋白1(MCP1)ELISA试剂盒 检测范围
zkA088Hu 单核细胞趋化蛋白2(MCP2)ELISA试剂盒 检测范围
zkA089Hu 单核细胞趋化蛋白3(MCP3)ELISA试剂盒 检测范围
zkA090Hu 巨噬细胞集落刺激因子(MCSF)ELISA试剂盒 检测范围
zkA091Hu 巨噬细胞来源趋化因子(MDC)ELISA试剂盒 检测范围
zkA102Hu 基质金属蛋白酶7(MMP7)ELISA试剂盒 检测范围
zkA103Hu 基质金属蛋白酶8(MMP8)ELISA试剂盒 检测范围
zkA105Hu 神经生长因子(NGF)ELISA试剂盒 检测范围
zkA106Hu 神经营养因子3(NT3)ELISA试剂盒 检测范围
zkA107Hu 神经营养因子4(NT4)ELISA试剂盒 检测范围
* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.通用Pan-Methyl组蛋白H3-K9定量试剂盒(Colorimetric) User Guide & MSDS
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