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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 英文名:
Fast Green FCF
- 保质期:
1年
- 供应商:
上海再康生物科技有限公司
- 保存条件:
RT
- 规格:
5g;25g
英文名称:Fast green FCF;Food green 3;C.I. 42053
其他名称:坚牢绿FCF;固绿;快绿;坚牢绿;食用色素绿色3号;食品绿3
CAS号:2353-45-9
C37H34N2Na2O10S3=808.85
级别:High Purity Grade
有效分光含量:≥90.0%
干燥失重:≤8.0%
Em (622nm, 50% Methanol):>125000
性状:深绿色粉末或栗色带金属光泽的颗粒。有吸湿性。易溶于水,溶于乙醇,溶于浓硫酸呈暗橙色,稀释后呈暗绿色,溶于浓盐酸或浓硝酸呈橙色,溶于10%溶液呈亮蓝色。熔点 290℃(分解)。最大吸收波长 628nm。半数致死量(大鼠,经口)>2G/kG
用途:生化研究。植物组织学和细胞学的染色。是一种碱性染料,用于IEF、PAGE 和 SDS-PAGE中的蛋白质染色。快绿染色比亮蓝R(Brilliant Blue R )覆盖更宽范围的蛋白质浓度,是线性的。 电泳过后,把蛋白质固定在胶上染色更加灵敏,在胶上用0.1%快绿溶于30% (v/v) 乙醇、10% (v/v) 醋酸或7% 醋酸染色2小时再用30% (v/v) 乙醇、10% (v/v) 乙酸或 7% 醋酸去染色. 染色胶能在 625 nm浏览。检测灵敏度要比亮蓝R(Brilliant Blue R)高30%。另外,快绿还用于亚硫酸盐测定,制造细菌培养基,用以鉴别大肠杆菌及其他乳糖发酵菌,培养分离粪便中的伤寒杆菌
保存:RT
Fast Green FCF, also called Food green 3, FD&C Green No. 3, Green 1724, Solid Green FCF, and C.I. 42053, is a sea green triarylmethane food dye. Its E number is E143.
Fast Green FCF is recommended as a replacement of Light Green SF yellowish in Masson's trichrome, as its color is more brilliant and less likely to fade. It is used as a quantitative stain for histones at alkaline pH after acid extraction of DNA. It is also used as a protein stain in electrophoresis. Its absorption maximum is at 625 nm.
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文献和实验() #交换 x 轴和 y 轴> p + ylim(0,15) #如果想截取某一段可以用此命令设置值域。> p + scale_x_continuous(breaks =seq(18,34,50) ) #设置刻度线位置> p + theme(axis.text.x = element_text(angle = 90,family = "Times",face = "italic",colour = "darkred",size=rel(0.9))) # angle = 90 设置字体角度, family
Whole genome amplification protocol for ChIP-chip
of ChIP samples (bottom panel). Oct4 binding sites confirmed by PCR are indicated with arrows. References Krig, S.R., Jin, V.X., Bieda, M., O'Geen, H., Green, R., and Farnham, P.J. (2007) Identification of genes directly regulated
Connective Tissue Stain for Muscle Biopsy :GOMORI TRICHROME
Chromotrope 2R 0.6g Fast green FCF 0.3g Phosphotungstic acid 0.6g Glacial acetic acid 1.0ml Distilled water 100ml Adjust to pH 3.4 METHOD Stain nuclei well (one minute
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