Connective Tissue Stain for Muscle Biopsy :GOMORI TRICHROME

Connective Tissue Stain for Muscle Biopsy :GOMORI TRICHROME

SECTIONS
Unfixed cryostat or cold Formol calcium/gum sucrose fixed sections on muscle biopsy.

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SOLUTIONS

Staining solution

• Chromotrope 2R 0.6g
  
• Fast green FCF 0.3g
  
• Phosphotungstic acid 0.6g
  
• Glacial acetic acid 1.0ml
  
• Distilled water 100ml
  
• Adjust to pH 3.4
  

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METHOD

 

1. Stain nuclei well (one minute) with haematoxylin and wash well in water.
   
2. Stain in staining solution for approximately 30 seconds-1 mintue.
   
3. Rinse in 0.2% acetic acid.
   
4. Dehydrate, clear . Mount sections in DPX
   

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RESULTS

Nemaline rods, ragged red fibres . Red on a blue/green background.

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NOTES
This method is virtually only used on muscle biopsies. The staining solution deteriorates with age, so if only used rarely it should be made up fresh each time

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