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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
pcDNA5/FRT/TO
- 保质期:
1年
- 供应商:
再康生物
- 保存条件:
低温运输,-20℃保存
质粒编号:
质粒名称: pcDNA5/FRT/TO
其他名称:
质粒大小(bp): 5137
抗性: Ampicillin
质粒类型: 哺乳动物细胞表达载体;cDNA表达载体;定向重组载体;四环素调控载体
是否病毒:非病毒
启动子:CMVTO(CMV/TetO2)
稳转或瞬转:瞬表达 或 稳表达
组成型或诱导性:诱导型
表达水平:高拷贝
蛋白标签:
5′ 测序引物及序列:CMV Forward: 5’-CGCAAATGGGCGGTAGGCGTG-3’
3′ 测序引物及序列:BGH Reverse: 5’-TAGAAGGCACAGTCGAGG-3’
GenBank号:
其他属性:pcDNA5/FRT/TO载体是cDNA的表达与克隆载体;
CMVTO启动子受四环素调控;
含有FRT位点(重组酶识别位点) ,与pOG44载体共转染,利用重组酶进行定向重组。
质粒图谱:
质粒序列:
| 1 | gacggatcgg | gagatctccc | gatcccctat | ggtgcactct | cagtacaatc | 50 |
| 51 | tgctctgatg | ccgcatagtt | aagccagtat | ctgctccctg | cttgtgtgtt | 100 |
| 101 | ggaggtcgct | gagtagtgcg | cgagcaaaat | ttaagctaca | acaaggcaag | 150 |
| 151 | gcttgaccga | caattgcatg | aagaatctgc | ttagggttag | gcgttttgcg | 200 |
| 201 | ctgcttcgcg | atgtacgggc | cagatatacg | cgttgacatt | gattattgac | 250 |
| 251 | tagttattaa | tagtaatcaa | ttacggggtc | attagttcat | agcccatata | 300 |
| 301 | tggagttccg | cgttacataa | cttacggtaa | atggcccgcc | tggctgaccg | 350 |
| 351 | cccaacgacc | cccgcccatt | gacgtcaata | atgacgtatg | ttcccatagt | 400 |
| 401 | aacgccaata | gggactttcc | attgacgtca | atgggtggag | tatttacggt | 450 |
| 451 | aaactgccca | cttggcagta | catcaagtgt | atcatatgcc | aagtacgccc | 500 |
| 501 | cctattgacg | tcaatgacgg | taaatggccc | gcctggcatt | atgcccagta | 550 |
| 551 | catgacctta | tgggactttc | ctacttggca | gtacatctac | gtattagtca | 600 |
| 601 | tcgctattac | catggtgatg | cggttttggc | agtacatcaa | tgggcgtgga | 650 |
| 651 | tagcggtttg | actcacgggg | atttccaagt | ctccacccca | ttgacgtcaa | 700 |
| 701 | tgggagtttg | ttttggcacc | aaaatcaacg | ggactttcca | aaatgtcgta | 750 |
| 751 | acaactccgc | cccattgacg | caaatgggcg | gtaggcgtgt | acggtgggag | 800 |
| 801 | gtctatataa | gcagagctct | ccctatcagt | gatagagatc | tccctatcag | 850 |
| 851 | tgatagagat | cgtcgacgag | ctcgtttagt | gaaccgtcag | atcgcctgga | 900 |
| 901 | gacgccatcc | acgctgtttt | gacctccata | gaagacaccg | ggaccgatcc | 950 |
| 951 | agcctccgga | ctctagcgtt | taaacttaag | cttggtaccg | agctcggatc | 1000 |
| 1001 | cactagtcca | gtgtggtgga | attctgcaga | tatccagcac | agtggcggcc | 1050 |
| 1051 | gctcgagtct | agagggcccg | tttaaacccg | ctgatcagcc | tcgactgtgc | 1100 |
| 1101 | cttctagttg | ccagccatct | gttgtttgcc | cctcccccgt | gccttccttg | 1150 |
| 1151 | accctggaag | gtgccactcc | cactgtcctt | tcctaataaa | atgaggaaat | 1200 |
| 1201 | tgcatcgcat | tgtctgagta | ggtgtcattc | tattctgggg | ggtggggtgg | 1250 |
| 1251 | ggcaggacag | caagggggag | gattgggaag | acaatagcag | gcatgctggg | 1300 |
| 1301 | gatgcggtgg | gctctatggc | ttctgaggcg | gaaagaacca | gctggggctc | 1350 |
| 1351 | tagggggtat | ccccacgcgc | cctgtagcgg | cgcattaagc | gcggcgggtg | 1400 |
| 1401 | tggtggttac | gcgcagcgtg | accgctacac | ttgccagcgc | cctagcgccc | 1450 |
| 1451 | gctcctttcg | ctttcttccc | ttcctttctc | gccacgttcg | ccggctttcc | 1500 |
| 1501 | ccgtcaagct | ctaaatcggg | ggctcccttt | agggttccga | tttagtgctt | 1550 |
| 1551 | tacggcacct | cgaccccaaa | aaacttgatt | agggtgatgg | ttcacgtacc | 1600 |
| 1601 | tagaagttcc | tattccgaag | ttcctattct | ctagaaagta | taggaacttc | 1650 |
| 1651 | cttggccaaa | aagcctgaac | tcaccgcgac | gtctgtcgag | aagtttctga | 1700 |
| 1701 | tcgaaaagtt | cgacagcgtc | tccgacctga | tgcagctctc | ggagggcgaa | 1750 |
| 1751 | gaatctcgtg | ctttcagctt | cgatgtagga | gggcgtggat | atgtcctgcg | 1800 |
| 1801 | ggtaaatagc | tgcgccgatg | gtttctacaa | agatcgttat | gtttatcggc | 1850 |
| 1851 | actttgcatc | ggccgcgctc | ccgattccgg | aagtgcttga | cattggggaa | 1900 |
| 1901 | ttcagcgaga | gcctgaccta | ttgcatctcc | cgccgtgcac | agggtgtcac | 1950 |
| 1951 | gttgcaagac | ctgcctgaaa | ccgaactgcc | cgctgttctg | cagccggtcg | 2000 |
| 2001 | cggaggccat | ggatgcgatc | gctgcggccg | atcttagcca | gacgagcggg | 2050 |
| 2051 | ttcggcccat | tcggaccgca | aggaatcggt | caatacacta | catggcgtga | 2100 |
| 2101 | tttcatatgc | gcgattgctg | atccccatgt | gtatcactgg | caaactgtga | 2150 |
| 2151 | tggacgacac | cgtcagtgcg | tccgtcgcgc | aggctctcga | tgagctgatg | 2200 |
| 2201 | ctttgggccg | aggactgccc | cgaagtccgg | cacctcgtgc | acgcggattt | 2250 |
| 2251 | cggctccaac | aatgtcctga | cggacaatgg | ccgcataaca | gcggtcattg | 2300 |
| 2301 | actggagcga | ggcgatgttc | ggggattccc | aatacgaggt | cgccaacatc | 2350 |
| 2351 | ttcttctgga | ggccgtggtt | ggcttgtatg | gagcagcaga | cgcgctactt | 2400 |
| 2401 | cgagcggagg | catccggagc | ttgcaggatc | gccgcggctc | cgggcgtata | 2450 |
| 2451 | tgctccgcat | tggtcttgac | caactctatc | agagcttggt | tgacggcaat | 2500 |
| 2501 | ttcgatgatg | cagcttgggc | gcagggtcga | tgcgacgcaa | tcgtccgatc | 2550 |
| 2551 | cggagccggg | actgtcgggc | gtacacaaat | cgcccgcaga | agcgcggccg | 2600 |
| 2601 | tctggaccga | tggctgtgta | gaagtactcg | ccgatagtgg | aaaccgacgc | 2650 |
| 2651 | cccagcactc | gtccgagggc | aaaggaatag | cacgtactac | gagatttcga | 2700 |
| 2701 | ttccaccgcc | gccttctatg | aaaggttggg | cttcggaatc | gttttccggg | 2750 |
| 2751 | acgccggctg | gatgatcctc | cagcgcgggg | atctcatgct | ggagttcttc | 2800 |
| 2801 | gcccacccca | acttgtttat | tgcagcttat | aatggttaca | aataaagcaa | 2850 |
| 2851 | tagcatcaca | aatttcacaa | ataaagcatt | tttttcactg | cattctagtt | 2900 |
| 2901 | gtggtttgtc | caaactcatc | aatgtatctt | atcatgtctg | tataccgtcg | 2950 |
| 2951 | acctctagct | agagcttggc | gtaatcatgg | tcatagctgt | ttcctgtgtg | 3000 |
| 3001 | aaattgttat | ccgctcacaa | ttccacacaa | catacgagcc | ggaagcataa | 3050 |
| 3051 | agtgtaaagc | ctggggtgcc | taatgagtga | gctaactcac | attaattgcg | 3100 |
| 3101 | ttgcgctcac | tgcccgcttt | ccagtcggga | aacctgtcgt | gccagctgca | 3150 |
| 3151 | ttaatgaatc | ggccaacgcg | cggggagagg | cggtttgcgt | attgggcgct | 3200 |
| 3201 | cttccgcttc | ctcgctcact | gactcgctgc | gctcggtcgt | tcggctgcgg | 3250 |
| 3251 | cgagcggtat | cagctcactc | aaaggcggta | atacggttat | ccacagaatc | 3300 |
| 3301 | aggggataac | gcaggaaaga | acatgtgagc | aaaaggccag | caaaaggcca | 3350 |
| 3351 | ggaaccgtaa | aaaggccgcg | ttgctggcgt | ttttccatag | gctccgcccc | 3400 |
| 3401 | cctgacgagc | atcacaaaaa | tcgacgctca | agtcagaggt | ggcgaaaccc | 3450 |
| 3451 | gacaggacta | taaagatacc | aggcgtttcc | ccctggaagc | tccctcgtgc | 3500 |
| 3501 | gctctcctgt | tccgaccctg | ccgcttaccg | gatacctgtc | cgcctttctc | 3550 |
| 3551 | ccttcgggaa | gcgtggcgct | ttctcatagc | tcacgctgta | ggtatctcag | 3600 |
| 3601 | ttcggtgtag | gtcgttcgct | ccaagctggg | ctgtgtgcac | gaaccccccg | 3650 |
| 3651 | ttcagcccga | ccgctgcgcc | ttatccggta | actatcgtct | tgagtccaac | 3700 |
| 3701 | ccggtaagac | acgacttatc | gccactggca | gcagccactg | gtaacaggat | 3750 |
| 3751 | tagcagagcg | aggtatgtag | gcggtgctac | agagttcttg | aagtggtggc | 3800 |
| 3801 | ctaactacgg | ctacactaga | agaacagtat | ttggtatctg | cgctctgctg | 3850 |
| 3851 | aagccagtta | ccttcggaaa | aagagttggt | agctcttgat | ccggcaaaca | 3900 |
| 3901 | aaccaccgct | ggtagcggtg | gtttttttgt | ttgcaagcag | cagattacgc | 3950 |
| 3951 | gcagaaaaaa | aggatctcaa | gaagatcctt | tgatcttttc | tacggggtct | 4000 |
| 4001 | gacgctcagt | ggaacgaaaa | ctcacgttaa | gggattttgg | tcatgagatt | 4050 |
| 4051 | atcaaaaagg | atcttcacct | agatcctttt | aaattaaaaa | tgaagtttta | 4100 |
| 4101 | aatcaatcta | aagtatatat | gagtaaactt | ggtctgacag | ttaccaatgc | 4150 |
| 4151 | ttaatcagtg | aggcacctat | ctcagcgatc | tgtctatttc | gttcatccat | 4200 |
| 4201 | agttgcctga | ctccccgtcg | tgtagataac | tacgatacgg | gagggcttac | 4250 |
| 4251 | catctggccc | cagtgctgca | atgataccgc | gagacccacg | ctcaccggct | 4300 |
| 4301 | ccagatttat | cagcaataaa | ccagccagcc | ggaagggccg | agcgcagaag | 4350 |
| 4351 | tggtcctgca | actttatccg | cctccatcca | gtctattaat | tgttgccggg | 4400 |
| 4401 | aagctagagt | aagtagttcg | ccagttaata | gtttgcgcaa | cgttgttgcc | 4450 |
| 4451 | attgctacag | gcatcgtggt | gtcacgctcg | tcgtttggta | tggcttcatt | 4500 |
| 4501 | cagctccggt | tcccaacgat | caaggcgagt | tacatgatcc | cccatgttgt | 4550 |
| 4551 | gcaaaaaagc | ggttagctcc | ttcggtcctc | cgatcgttgt | cagaagtaag | 4600 |
| 4601 | ttggccgcag | tgttatcact | catggttatg | gcagcactgc | ataattctct | 4650 |
| 4651 | tactgtcatg | ccatccgtaa | gatgcttttc | tgtgactggt | gagtactcaa | 4700 |
| 4701 | ccaagtcatt | ctgagaatag | tgtatgcggc | gaccgagttg | ctcttgcccg | 4750 |
| 4751 | gcgtcaatac | gggataatac | cgcgccacat | agcagaactt | taaaagtgct | 4800 |
| 4801 | catcattgga | aaacgttctt | cggggcgaaa | actctcaagg | atcttaccgc | 4850 |
| 4851 | tgttgagatc | cagttcgatg | taacccactc | gtgcacccaa | ctgatcttca | 4900 |
| 4901 | gcatctttta | ctttcaccag | cgtttctggg | tgagcaaaaa | caggaaggca | 4950 |
| 4951 | aaatgccgca | aaaaagggaa | taagggcgac | acggaaatgt | tgaatactca | 5000 |
| 5001 | tactcttcct | ttttcaatat | tattgaagca | tttatcaggg | ttattgtctc | 5050 |
| 5051 | atgagcggat | acatatttga | atgtatttag | aaaaataaac | aaataggggt | 5100 |
| 5101 | tccgcgcaca | tttccccgaa | aagtgccacc | tgacgtc | 5137 |
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