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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pAB520
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-80℃保存
质粒编号:
质粒名称: pAB520
其他名称:
质粒大小（bp）: 4100
抗性:
质粒类型: Unspecified
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性: That expression was improved by modifying the 17 bp before the initiation codon in pAB366 to match the sequence of the am gene. Shuttle vector conferring bleomycin and phleomycin resistance. Expression of ble (bleomycin resistance) is regulated by the promoter and terminator regions of the am gene (amination, glutamate dehydrogenase) from N. crassa. The am::ble fusion cassette can be removed by digestion with either HindIII, SphI, XbaI or BamHI or any combination of them. The order of the major features in this plasmid is:pUC18 – MCS (HindIII to BamHI) – am promoter – ble – XbaI – am 3' region – EcoRI – MCS (HindIII to EcoRI) – pUC18. Restriction digests of the clone give the following sizes (kb): HindIII–2.7, 1.4; KpnI–4.1; SmaI–3.1, 1.0. (ATCC staff) A (GT)n repeat. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Aspergillus nidulans, Neurospora crassa, E.coli.
质粒图谱:
质粒序列:
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