MIGS4.2 (FF575)

质粒编号:
质粒名称: MIGS4.2 (FF575)
其他名称:
质粒大小（bp）: 8328
抗性: Spectinomycin
质粒类型: plant expression
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性: Growth strain(s)：ccdB Survival
Growth temperature (℃):37
Growth instructions:
1) Before recombination Chloramphenicol might be used for bacteria selection (resistance lost after recombination). 2) E. coli DB3.1 strain (or some other ccdB survival strain) should be used for the amplification of the plasmid due to the presence of the lethal ccdB gene. After recombination, any common strain, such as DH5alpha is recommended. 3) Due to pGreen backbone characteristics, the helper plasmid pSOUP is required for replication of MIGS vector in Agrobacterium. PSOUP can be requested directly at
High or low copy:High Copy
Selectable markers, other: kanamycin
质粒图谱:

质粒序列:

>MIGS4.2 (FF575) sequence 8328 bps
TTTTTATCCCCGGAAGCCTGTGGATAGAGGGTAGTTATCCACGTGAAACCGCTAATGCCCCGCAAAGCCT
TGATTCACGGGGCTTTCCGGCCCGCTCCAAAAACTATCCACGTGAAATCGCTAATCAGGGTACGTGAAAT
CGCTAATCGGAGTACGTGAAATCGCTAATAAGGTCACGTGAAATCGCTAATCAAAAAGGCACGTGAGAAC
GCTAATAGCCCTTTCAGATCAACAGCTTGCAAACACCCCTCGCTCCGGCAAGTAGTTACAGCAAGTAGTA
TGTTCAATTAGCTTTTCAATTATGAATATATATATCAATTATTGGTCGCCCTTGGCTTGTGGACAATGCG
CTACGCGCACCGGCTCCGCCCGTGGACAACCGCAAGCGGTTGCCCACCGTCGAGCGCCAGCGCCTTTGCC
CACAACCCGGCGGCCGGCCGCAACAGATCGTTTTATAAATTTTTTTTTTTGAAAAAGAAAAAGCCCGAAA
GGCGGCAACCTCTCGGGCTTCTGGATTTCCGATCCCCGGAATTAGAGATCTTGGCAGGATATATTGTGGT
GTAACGTTATCAGCTTGCATGCCGGTCGATCTAGTAACATAGATGACACCGCGCGCGATAATTTATCCTA
GTTTGCGCGCTATATTTTGTTTTCTATCGCGTATTAAATGTATAATTGCGGGACTCTAATCATAAAAACC
CATCTCATAAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTATA
TGATAATCATCGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGATC
TGCTTGACTCTAGGGGTCATCAGAAGAACTCGTCAAGAAGGCGATAGAAGGCGATGCGCTGCGAATCGGG
AGCGGCGATACCGTAAAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGCAATATCACGG
GTAGCCAACGCTATGTCCTGATAGCGGTCCGCCACACCCAGCCGGCCACAGTCGATGAATCCAGAAAAGC
GGCCATTTTCCACCATGATATTCGGCAAGCAGGCATCGCCCTGGGTCACGACGAGATCCTCGCCGTCGGG
CATCCGCGCCTTGAGCCTGGCGAACAGTTCGGCTGGCGCGAGCCCCTGATGCTCTTCGTCCAGATCATCC
TGATCGACAAGACCGGCTTCCATCCGAGTACGTCCTCGCTCGATGCGATGTTTCGCTTGGTGGTCGAATG
GGCAGGTAGCCGGATCAAGCGTATGCAGCCGCCGCATTGCATCAGCCATGATGGATACTTTCTCGGCAGG
AGCAAGGTGAGATGACAGGAGATCCTGCCCCGGCACTTCGCCCAATAGCAGCCAGTCCCTTCCCGCTTCA
GTGACAACGTCGAGCACAGCTGCGCAAGGAACGCCCGTCGTGGCCAGCCACGATAGCCGCGCTGCCTCGT
CTTGGAGTTCATTCAGGGCACCGGACAGGTCGGTCTTGACAAAAAGAACCGGGCGCCCCTGCGCTGACAG
CCGGAACACGGCGGCATCAGAGCAGCCGATTGTCTGTTGTGCCCAGTCATAGCCGAATAGCCTCTCCACC
CAAGCGGCCGGAGAACCTGCGTGCAATCCATCTTGTTCAATCATGCCTCGATCGAGTTGAGAGTGAATAT
GAGACTCTAATTGGATACCGAGGGGAATTTATGGAACGTCAGTGGAGCATTTTTGACAAGAAATATTTGC
TAGCTGATAGTGACCTTAGGCGACTTTTGAACGCGCAATAATGGTTTCTGACGTATGTGCTTAGCTCATT
AAACTCCAGAAACCCGCGGATTTAGGTGACACTATAGAATATCACTAGTAAGCTAGCTTGCATGCCGGTC
CTGCTGAGCCTCGACATGTTGTCGCAAAATTCGCCCTGGACCCGCCCAACGATTTGTCGTCACTGTCAAG
GTTTGACCTGCACTTCATTTGGGGCCCACATACACCAAAAAAATGCTGCATAATTCTCGGGGCAGCAAGT
CGGTTACCCGGCCGCCGTGCTGGACCGGGTTGAATGGTGCCCGTAACTTTCGGTAGAGCGGACGGCCAAT
ACTCAACTTCAAGGAATCTCACCCATGCGCGCCGGCGGGGAACCGGAGTTCCCTTCAGTGAGCGTTATTA
GTTCGCCGCTCGGTGTGTCGTAGATACTAGCCCCTGGGGCACTTTTGAAATTTGAATAAGATTTATGTAA
TCAGTCTTTTAGGTTTGACCGGTTCTGCCGCTTTTTTTAAAATTGGATTTGTAATAATAAAACGCAATTG
TTTGTTATTGTGGCGCTCTATCATAGATGTCGCTATAAACCTATTCAGCACAATATATTGTTTTCATTTT
AATATTGTACATATAAGTAGTAGGGTACAATCAGTAAATTGAACGGAGAATATTATTCATAAAAATACGA
TAGTAACGGGTGATATATTCATTAGAATGAACCGAAACCGGCGGTAAGGATCTGAGCTACACATGCTCAG
GTTTTTTACAACGTGCACAACAGAATTGAAAGCAAATATCATGCGATCATAGGCGTCTCGCATATCTCAT
TAAAGCAGGGATCCCGCATCAGATCTATCAAACACATAGATTTACGAAGATCTCTAACATTAAATCATCA
GAGAGATAAGTTTAGGGAGCAAGCTCTTTCGCTTACACAGAGAATCACAGAGGAAAGAGACTTTAAGAAA
ACTACAACTTTTTTGACCACTGTGATTTCTCTCTGCAAGCGAAAGTACTTAATCACCAACACAGTAGATT
GATATATTTGGGTTTATTTGTGAGAAGAAGAATTCACTAGTGGATCCCGCACTCGAGTGTTAATCAGAAA
AACTCAGATTAATCTACAAATTCGATCGCACAAACTAGAAACTAACACCAGATCTAGATAGAAATCACAA
ATCGAAGAGTAATTATTCGACAAAACTCAAATTATTTGAACAAATCGGATGATATCTATGAAACCCTAAT
CGAGAATTAAGATGATATCTAACGATCAAACCCAGAAAATCGTCTTCGATCTAAGATTAACAGAATCTAA
ACCAAAGAACATATACGAAATTGGGATCGAACGAAAACAAAATCGAAGATTTTGAGAGAATAAGGAACAC
AGAAATTTACCTTGATCACGGTAGAGAGAATTGAGAGAAAGTTTTTAAGATTTTGAGAAATTGAAATCTG
AATTGTGAAGAAGAAGAGCTCTTTGGGTATTGTTTTATAGAAGAAGAAGAAGAAAAGACGAGGACGACTA
GGTCACGAGAAAGCTAAGGCGGTGAAGCAATAGCTAATAATAAAATGACACGTGTATTGAGCGTTGTTTA
CACGCAAAGTTGTTTTTGGCTAATTGCCTTATTTTTAGGTTGAGGAAAAGTATTTGTGCTTTGAGTTGAT
AAACACGACTCGTGTGTGCCGGCTGCAACCACTTTGACGCCGTTTATTACTGACTCGTCGGGTACCGGGC
CCCCCCTCGAGGTCGACGGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCGGGGGATCCACTAGTTC
TAGAGGATCACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAA
ATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCATATTGGCGGCC
CCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGAGC
CGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATAT
ATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACC
GTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTA
TTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAGTTCCGTATGGCAATGAAAGACGGTGAGCTGGT
GATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGG
AGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAA
ACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTT
CACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATAT
TATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCC
ATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGG
AGCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATAT
ACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGACAGTTGA
CAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGC
AGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGC
CCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTA
CACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGC
CGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGG
TGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTAT
CGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGG
GGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCACCTCGACCATAGTGACTGGATATGTTGT
GTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTT
ACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATCCTAGCTTTCGTTCGTATCATCGGTTTCGACAA
CGTTCGTCAAGTTCAATGCATCAGTTTCATTGCGCACACACCAGAATCCTACTGAGTTTGAGTATTATGG
CATTGGGAAAACTGTTTTTCTTGTACCATTTGTTGTGCTTGTAATTTACTGTGTTTTTTATTCGGTTTTC
GCTATCGAACTGTGAAATGGAAATGGATGGAGAAGAGTTAATGAATGATATGGTCCTTTTGTTCATTCTC
AAATTAATATTATTTGTTTTTTCTCTTATTTGTTGTGTGTTGAATTTGAAATTATAAGAGATATGCAAAC
ATTTTGTTTTGAGTAAAAATGTGTCAAATCGTGGCCTCTAATGACCGAAGTTAATATGAGGAGTAAAACA
CTTGTAGTTGTACCATTATGCTTATTCACTAGGCAACAAATATATTTTCAGACCTAGAAAAGCTGCAAAT
GTTACTGAATACAAGTATGTCCTCTTGTGTTTTAGACATTTATGAACTTTCCTTTATGTAATTTTCCAGA
ATCCTTGTCAGATTCTAATCATTGCTTTATAATTATAGTTATACTCATGGATTTGTAGTTGAGTATGAAA
ATATTTTTTAATGCATTTTATGACTTGCCAATTGATTGACAACATGCATCAATCGGAGCTCCAGCTTTTG
TTCCCTTTAGTGAGGGTTAATTCCGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGT
TATCCGCTCACAATTCCACACAACATACGAGCCGGAAGHCATAAAGTGTAAAGCCTGGGGTGCCTAATGA
GTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGC
TGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCT
CACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGG
TTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGAAGGCCTTGACAGGATATATTGGCGGGTAAA
CTAAGTCGCTGTATGTGTTTGTTTGAGATCTCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAA
AAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCA
AGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCT
TTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCAC
GAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTAC
AGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTG
AAGCCAGTTACCTTCGGAAGAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTC
TACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGG
ATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGTGTAACATT
GGTCTAGTGATTATTTGCCGACTACCTTGGTGATCTCGCCTTTCACGTAGTGAACAAATTCTTCCAACTG
ATCTGCGCGCGAGGCCAAGCGATCTTCTTGTCCAAGATAAGCCTGCCTAGCTTCAAGTATGACGGGCTGA
TACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGCGACATCCTTCGGCGCGATTTTGCCGGTTACTG
CGCTGTACCAAATGCGGGACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAGTT
CCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGGAACCGGATCAAAGAGTTCCTCC
GCCGCTGGACCTACCAAGGCAACGCTATGTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGA
TCGTGGCTGGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTGCAGTTCGCGCTT
AGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAACAATGGTGACTTCTACAGCGCGGAGAATCTCG
CTCTCTCCAGGGGAAGCCGAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAGCC
TTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGCCGCCATCCACTGCGGAGCCGTA
CAAATGTACGGCCAGCAACGTCGGTTCGAGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTC
GATACTTCGGCGATCACCGCTTCCCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTA
TTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTG
TTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACGCATCTTCCCGACAACGCAGACCGTTCC
GTGGCAAAGCAAAAGTTCAAAATCACCAACTGGTCCACCTACAACAAAGCTCTCATCAACCGTGGCTCCC
TCACTTTCTGGCTGGATGATGGGGCGATTCAGGCGATCCCCATCCAACAGCCCGCCGTCGAGCGGGCT
>SP6
GGTCCTGCTGAGCCTCGACATGTTGTCGCAAAATTCGCCCTGGACCCGCCCAACGATTTGTCGTCACTGT
CAAGGTTTGACCTGCACTTCATTTGGGGCCCACATACACCAAAAAAATGCTGCATAATTCTCGGGGCAGC
AAGTCGGTTACCCGGCCGCCGTGCTGGACCGGGTTGAATGGTGCCCGTAACTTTCGGTAGAGCGGACGGC
CAATACTCAACTTCAAGGAATCTCACCCATGCGCGCCGGCGGGGAACCGGAGTTCCCTTCAGTGAGCGTT
ATTAGTTCGCCGCTCGGTGTGTCGTAGATACTAGCCCCTGGGGCACTTTTGAAATTTGAATAAGATTTAT
GTAATCAGTCTTTTAGGTTTGACCGGTTCTGCCGCTTTTTTTAAAATTGGATTTGTAATAATAAAACGCA
ATTGTTTGTTATTGTGGCGCTCTATCATAGATGTCGCTATAAACCTATTCAGCACAATATATTGTTTTCA
TTTTAATATTGTACATATAAGTAGTAGGGTACAATCAGTAAATTGAACGGAGAATATTATTCATAAAAAT
ACGATAGTAACGGGTGATATATTCATTAGAATGAACCGAAACCGGCGGTAAGGATCTGAGCTACACATGC
TCAGGTTTTTTACAACGTGCACAACAGAATTGAAAGCAAATATCATGCGATCATAGGCGTCTCGCATATC
TCATTAAAGCAGGGATCCCGCATCAGATCTATCAAACACATAGATTTACGAAGATCTCTAACATTAAATC
ATCAGAGAGATAAGTTTAGGGAGCAAGCTCTTTCGCTTACACAGAGAATCACAGAGGAAAGAGACTTTAA
GAAAACTACAACTTTTTTGACCACTGTGATTTCTCTCTGC
>CAT-R
TTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGACGGCTCCTAACTCAAAATCCACACATT
ATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGGGGCCGCCAATATGACTGGATATGTT
GTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATT
TTACGTTTCTCGTTCAGCTTTTTTGTACAAACTTGTGATCCTCTAGAACTAGTGGATCCCCCGGGCTGCA
GGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAGGGGGGGCCCGGTACCCGACGAGTCAGTAAT
AAACGGCGTCAAAGTGGTTGCAGCCGGCACACACGAGTCGTGTTTATCAACTCAAAGCACAAATACTTTT
CCTCAACCTAAAAATAAGGCAATTAGCCAAAAACAACTTTGCGTGTAAACAACGCTCAATACACGTGTCA
TTTTATTATTAGCTATTGCTTCACCGCCTTAGCTTTCTCGTGACCTAGTCGTCCTCGTCTTTTCTTCTTC
TTCTTCTATAAAACAATACCCAAAGAGCTCTTCTTCTTCACAATTCAGATTTCAATTTCTCAAAATCTTA
AAAACTTTCTCTCAATTCTCTCTACCGTGATCAAGGTAAATTTCTGTGTTCCTTATTCTCTCAAAATCTT
CGA