- 询价
- R&D
- ZK-0002970
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- GFAP (6F2) Monoclonal Antibody, Purified (SIGNET)详细信息：
-
GFAP (6F2) Monoclonal Antibody, Purified (SIGNET)Description Monoclonal Antibody against GFAP
(Formerly Signet Catalog Nos: 470-01, 470-16 L1 Predilute, 470-26 L2 Predilute)Intended Use ** In Vitro Diagnostic (IVD) ** Clone 6F2 Form Tissue Culture Supernatant (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host Mouse Species Reactivity Human IsoType GFAP (6F2) Monoclonal Antibody, Purified (SIGNET)IgG1 Specificity The GFAP monoclonal antibody recognizes a 52KDa protein and stains normal glial cells as well as astrocytomas and ependymomas. It does not react with vimentin, neurofilament, cytokeratin, or desmin.
This antibody was raised against intermediate filament of bovine spinal cord.
Concentrated Format:
SIG-3470-1000 [1 mL]
Prediluted Formats:
SIG-3470-16 [6 mL] is ready to use with Peroxidase Anti- Peroxidase detection systems.
SIG-3470-26 [6 mL] is ready-to-use with Biotin-based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).Uses GFAP (6F2) Monoclonal Antibody, Purified (SIGNET)This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as USA Ultra Sreptavidin Detection (SIG-32250).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: Not required
Incubation: 20 minutes at room temperature
Notes Positive tissue (human): Brain (cerebellum) - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as USA Ultra Sreptavidin Detection (SIG-32250).
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文献和实验Monoclonal Antibody Affinity Chromatography
all the above criteria and also afford the advantage of being available in essentially unlimited supply. Moreover, they are themselves readily purified, often by affinity chromatography. The main disadvantage of monoclonal antibody ligands is that they must possess
Antigen Purification by Monoclonal Antibody Immunoaffinity Chromatography
proteins. Taking advantage of the exquisite specificity afforded by each antibody results in a final purified product that binds specifically to that MAb. Using immunoaffinity chromatography, a single antigen can be separated from very complex mixtures (e.g
Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies
Repertoires of antibody (Ab) V genes derived from nonimmunized human donors (1 ) or made synthetically (2 3 ) have been cloned for display on filamentous bacteriophage as either scFvs or Fabs fused to the minor phage coat protein (pIII
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