Invitrogen A-21206 Donkey anti
-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488- ￥3740
- Invitrogen
- A-21206
- 美国
- 2025年12月14日
- 免疫组化（石蜡） (IHC (P))，免疫组化（冰冻） (IHC (F))，免疫细胞化学 (ICC/IF)，流式细胞分析 (Flow)
- 驴
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
驴抗兔 IgG（H+L）高度交叉吸附二抗，Alexa Fluor™ 488 标记
- 抗体英文名：
Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488
- 靶点：
特异性识别兔 IgG 的重链和轻链（经高度交叉吸附，降低与其他种属免疫球蛋白的非特异性结合）
- 浓度：
2 mg/mL
- 应用范围：
免疫组化（石蜡） (IHC (P))，免疫组化（冰冻） (IHC (F))，免疫细胞化学 (ICC/IF)，流式细胞分析 (Flow)
- 宿主：
驴
- 供应商：
上海然其生物科技有限公司
- 库存：
现货
- 级别：
见包装
- 目录编号：
无
- 保质期：
见包装
- 标记物：
偶联Alexa Fluor™ 488绿色荧光染料，激发波长约 495 nm，发射波长约 519 nm，亮度高、光稳定性强，适配荧光显微镜、流式细胞仪等设备的绿色荧光通道。
- 克隆性：
多克隆
- 保存条件：
4℃避光保存，严禁冷冻，避免反复冻融
- 形态：
液体
产品详细信息
To minimize cross-reactivity, these donkey anti-rabbit IgG whole antibodies have been affinity-purified and show minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.
Product will be shipped at Room Temperature.
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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