Invitrogen A-11055 Donkey anti
-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488- ￥3758
- Invitrogen
- A-11055
- 美国
- 2025年12月14日
- 免疫组织化学（IHC）免疫细胞化学 / 免疫荧光（ICC/IF）流式细胞术（Flow
- 驴
- 兔、大鼠、小鼠和人源
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488
- 靶点：
山羊的 IgG（重链 + 轻链，即 H+L）
- 浓度：
2mg/mL
- 应用范围：
免疫组织化学（IHC）免疫细胞化学 / 免疫荧光（ICC/IF）流式细胞术（Flow
- 宿主：
驴
- 供应商：
上海然其生物科技有限公司
- 库存：
现货
- 级别：
见包装
- 目录编号：
无
- 抗原来源：
山羊免疫球蛋白的重链和轻链复合物
- 保质期：
见包装
- 适应物种：
兔、大鼠、小鼠和人源
- 标记物：
Alexa Fluor™ 488，该染料为明亮的绿色荧光染料，激发波长峰值约 499nm，发射波长峰值约 520nm，适配 488nm 激光线，且在较宽 pH 范围内不敏感，光稳定性强、荧光量子产率高，能实现低丰度靶点的高灵敏度检测
- 克隆性：
多克隆
- 保存条件：
4℃避光保存，严禁冷冻，避免反复冻融
- 形态：
液体
- 亚型：
IgG
- 规格：
1 mg
产品详细信息
To minimize cross-reactivity, these donkey anti-goat IgG whole antibodies have been cross-adsorbed against rabbit, rat, mouse, and human IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins.
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.
Product will be shipped at Room Temperature.
靶标信息
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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