Invitrogen A21203 Donkey anti-
Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594- ￥3740
- Invitrogen
- A21203
- 美国
- 2025年12月14日
- 适用于免疫荧光（IF）、免疫细胞化学（ICC）、流式细胞分析（Flow Cytometry）、免疫组织化学（IHC）等荧光检测实验
- 驴
- 小鼠（经高度交叉吸附处理，大幅降低与其他种属免疫球蛋白的非特异性结合）
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594
- 抗体英文名：
Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594
- 靶点：
无特定靶点（作为二抗，靶向大鼠 IgG 重链和轻链）
- 浓度：
2 mg/mL
- 应用范围：
适用于免疫荧光（IF）、免疫细胞化学（ICC）、流式细胞分析（Flow Cytometry）、免疫组织化学（IHC）等荧光检测实验
- 宿主：
驴
- 供应商：
上海然其生物科技有限公司
- 库存：
现货
- 级别：
见包装
- 目录编号：
无
- 抗原来源：
驴
- 保质期：
见包装
- 适应物种：
小鼠（经高度交叉吸附处理，大幅降低与其他种属免疫球蛋白的非特异性结合）
- 标记物：
Alexa Fluor™ 594（红色荧光染料，激发波长～590 nm，发射波长～617 nm，亮度高、光稳定性强，抗淬灭性能优异）
- 克隆性：
多克隆
- 保存条件：
4℃环境且避光保存，避免反复冻融
- 形态：
液体
- 亚型：
IgG
- 免疫原：
小鼠 IgG
产品详细信息
These donkey anti-mouse IgG (H+L) whole secondary antibodies have been affinity-purified and show minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 594 dye is a bright, red-fluorescent dye with excitation ideally suited to the 594 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 594 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.
Product will be shipped at Room Temperature.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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