- ￥1114
- Invitrogen
- 12-4998-82
- 美国
- 2025年12月14日
- 流式细胞术（Flow Cytometry），可用于人类 IgG 相关的细胞表型分析、免疫细胞分选等实验，推荐单次实验用量 1μg，实际使用可根据实验需求滴定浓度。
- 山羊
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
羊抗人 IgG Fc 段二抗，PE 标记
- 抗体英文名：
Goat anti-Human IgG Fc Secondary Antibody, PE, eBioscience™
- 靶点：
特异性识别人类 IgG 的 Fc 片段，不结合轻链、IgM、IgA 及非免疫球蛋白血清蛋白
- 浓度：
0.5mg/mL
- 应用范围：
流式细胞术（Flow Cytometry），可用于人类 IgG 相关的细胞表型分析、免疫细胞分选等实验，推荐单次实验用量 1μg，实际使用可根据实验需求滴定浓度。
- 宿主：
山羊
- 供应商：
上海然其生物科技有限公司
- 库存：
现货
- 级别：
见包装
- 目录编号：
无
- 保质期：
见包装
- 标记物：
偶联 PE（藻红蛋白）荧光染料，激发波长约 565nm，发射波长约 576nm，适配主流流式细胞仪激光通道，荧光亮度高、信号稳定，适合流式细胞分析等荧光检测场景。
- 克隆性：
多克隆
- 保存条件：
4℃避光保存，严禁冷冻，避免反复冻融
- 形态：
液体（含 0.2μm 过滤处理）
产品详细信息
Description: This goat antibody reacts with the Fc fragment of heavy chains on human IgG but notwith the light chains on most human immunoglobulins. No antibody was detected against human IgM or IgA, or against nonimmunoglobulinserum proteins. This antibody has been tested to ensure minimalcross-reaction with bovine, horse, and mouse serum proteins, but the antibody may cross-react with immunoglobulins fromother species.
Applications Reported: PE goat anti-Human IgG (Fc gamma Fragment Specific) has been reported for use in flow cytometric analysis.
Applications Tested: PE goat anti-Human IgG (Fc gamma Fragment Specific) has been tested by flow cytometric analysis to detect purified human Fc gamma-tagged recombinant proteins. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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