- ￥10480
- Sino Biological
- 北京
- 11007-H73H-NB-B
- 2025年11月14日
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- 详细信息
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CD20 Protein (Nanodisc, AVI & His Tag), Biotinylated
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20 µg
重组人 CD20 蛋白 (Nanodisc, AVI & His Tag), Biotinylated（产品说明）
蛋白名称：Human CD20 Protein (Nanodisc, AVI & His Tag), Biotinylated
蛋白构建：A DNA sequence encoding the Human MS4A1 (P11836) (Met1-Pro297) was expressed with a AVI tag at the N-terminus, a polyhistidine tag at the C-terminus.The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 75 % as determined by SDS-PAGE.
蛋白活性：1. Immobilized Recombinant Human CD20 Protein (Nanodisc, AVI & His Tag), Biotinylated (Cat: 11007-H73H-NB-B) at 5 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-CD20 (Research Grade Obinutuzu*mab Biosimilar), the EC50 is 3.5-10.5 ng/mL (QC tested). 2. Immobilized Recombinant Human CD20 Protein (Nanodisc, AVI & His Tag), Biotinylated (Cat: 11007-H73H-NB-B) at 5 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-CD20 (Research Grade Rituxi*mab Biosimilar), the EC50 is 5-15 (Routinely tested). 3. Immobilized Anti-CD20 (Research Grade Obinutuzu*mab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD20 Protein (Nanodisc, AVI & His Tag), Biotinylated (Cat: 11007-H73H-NB-B), the EC50 is 0.5-2 ng/mL (QC tested). 4. Immobilized Anti-CD20 (Research Grade Rituxi*mab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD20 Protein (Nanodisc, AVI & His Tag), Biotinylated (Cat: 11007-H73H-NB-B), the EC50 is 1-5 ng/mL (QC tested). 5. Immobilized Recombinant Human CD3E & CD3G Heterodimer Protein, Low Endotoxin (Cat: CT089-H46H-E) at 1 μg/mL (100 μL/well), add Anti-CD20/CD3 (Research Grade Glofita*mab Biosimilar) and then add Recombinant Human CD20 Protein (Nanodisc, AVI & His Tag), Biotinylated (Cat: 11007-H73H-NB-B) at 0.1 μg/mL (100 μL/well). Detection was performed using HRP Streptavidin , the EC50 is 20-60 ng/mL (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Met 1
蛋白分子量：The recombinant Human CD20 comprises 338 amino acids and has a predicted molecular mass of 37.34 kDa. It migrates as an approximately 41.4 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：P11836
蛋白氨基酸序列：Met1-Pro297
蛋白名称：Human CD20 Protein (Nanodisc, AVI & His Tag), Biotinylated
蛋白构建：A DNA sequence encoding the Human MS4A1 (P11836) (Met1-Pro297) was expressed with a AVI tag at the N-terminus, a polyhistidine tag at the C-terminus.The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 75 % as determined by SDS-PAGE.
蛋白活性：1. Immobilized Recombinant Human CD20 Protein (Nanodisc, AVI & His Tag), Biotinylated (Cat: 11007-H73H-NB-B) at 5 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-CD20 (Research Grade Obinutuzu*mab Biosimilar), the EC50 is 3.5-10.5 ng/mL (QC tested). 2. Immobilized Recombinant Human CD20 Protein (Nanodisc, AVI & His Tag), Biotinylated (Cat: 11007-H73H-NB-B) at 5 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-CD20 (Research Grade Rituxi*mab Biosimilar), the EC50 is 5-15 (Routinely tested). 3. Immobilized Anti-CD20 (Research Grade Obinutuzu*mab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD20 Protein (Nanodisc, AVI & His Tag), Biotinylated (Cat: 11007-H73H-NB-B), the EC50 is 0.5-2 ng/mL (QC tested). 4. Immobilized Anti-CD20 (Research Grade Rituxi*mab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD20 Protein (Nanodisc, AVI & His Tag), Biotinylated (Cat: 11007-H73H-NB-B), the EC50 is 1-5 ng/mL (QC tested). 5. Immobilized Recombinant Human CD3E & CD3G Heterodimer Protein, Low Endotoxin (Cat: CT089-H46H-E) at 1 μg/mL (100 μL/well), add Anti-CD20/CD3 (Research Grade Glofita*mab Biosimilar) and then add Recombinant Human CD20 Protein (Nanodisc, AVI & His Tag), Biotinylated (Cat: 11007-H73H-NB-B) at 0.1 μg/mL (100 μL/well). Detection was performed using HRP Streptavidin , the EC50 is 20-60 ng/mL (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Met 1
蛋白分子量：The recombinant Human CD20 comprises 338 amino acids and has a predicted molecular mass of 37.34 kDa. It migrates as an approximately 41.4 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：P11836
蛋白氨基酸序列：Met1-Pro297
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重组人 CD20 蛋白 (Nanodisc, AVI & His Tag), Biotinylated
￥10480
