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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
IL-2 receptor subunit alpha| IL-2-RA| IL-2R subunit alpha| IL2-RA| IL2RA| Interleukin-2 receptor subunit alpha| TAC antigen| p55
- 亚型:
IgG1, κ
- 形态:
liquid
- 保存条件:
PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
- 克隆性:
Monoclonal
- 标记物:
PE
- 适应物种:
Mouse
- 保质期:
12个月
- 级别:
科研
- 库存:
100
- 供应商:
武汉菲恩生物科技有限公司
- 宿主:
Rat
- 应用范围:
FC
- 靶点:
Mouse CD25
- 抗体英文名:
PE Anti-Mouse CD25 Antibody(PC-61.5.3)
- 抗体名:
PE Anti-Mouse CD25 Antibody(PC-61.5.3)
- 规格:
25μg/100μg
| 规格: | 25μg | 产品价格: | ¥560.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥1820.0 |

产品介绍
| 产品名称 | PE Anti-Mouse CD25 Antibody(PC-61.5.3) |
| 货号 | PE-30017U |
| 规格 | 25μg/100μg |
| 宿主 | Rat |
| 反应种属 | Mouse |
| 验证应用 | FC |
| 状态 | liquid |
| 偶联物 | PE |
| 克隆性 | Monoclonal |
| 亚型 | IgG1, κ |
| Clone ID | PC-61.5.3 |
| 免疫原 | IL-2 receptor subunit alpha| IL-2-RA| IL-2R subunit alpha| IL2-RA| IL2RA| Interleukin-2 receptor subunit alpha| TAC antigen| p55 |
| Uniprot ID | P01590 |
| 验证图片 | ![]() |
| 存储 | PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
| 建议稀释比例 | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1μg/10⁶ cells in 100 μL volume]. |
| 注意事项 | PE Anti-Mouse CD25 Antibody(PC-61.5.3)仅供科研使用 |
流式抗体是一类专门用于流式细胞术的抗体,通过与荧光标记物结合,从而实现对细胞表面或内部的特定蛋白质进行精确检测和定量分析。流式抗体广泛应用于生物医学研究、临床诊断、药物开发和生物工程等领域。菲恩生物基于自身单抗开发平台,推出亲和力高,特异性好的经典克隆细胞株纯化抗体,产品涵盖人、大鼠、小鼠等,指标丰富,可选择性多。同时菲恩生物提供免费的配色,实验指导和数据分析服务,为您提供整体流式解决方案。
18种荧光标记, 满足多色流式实验需求。 采用进口荧光染料, 有更好的荧光亮度。

数据展示

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文献和实验T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
1. Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is used. 2. Aspirate media. Rinse cells with PBS, aspirate.3. Lyse cells in 200 uL of SDS sample buffer (100 ℃). Immediately scrape the cells off the plate with a rubber
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
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