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.niox.2009.01.002 (2009). 11.  Xiao, Y. & Truskey, G.A. Effect of receptor-ligand affinity on the strength of endothelial cell adhesion. Biophys. J. 71, 2869-2884, doi:10.1016/S0006-3495(96)79484-5 (1996). 12.  Conant, C.G., Schwartz, M

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两次后，加入200ul缓冲液[2% Triton X-100, 1% SDS, 100 mM NaCl, 10 mM Tris-HCl (pH 8.0), 1 mM EDTA (pH 8.0)]，使用液氮和96-98度沸水反复冻融3-5次，然后使用酚氯仿抽提蛋白!其余步骤参考其他文献 3.下面两个文献对你会有所帮助!请参考!不错的方法! For yeast plasmid extraction we use the following method:

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over the years, but they have never caught on in a big way. They are the sort of things used when more ordinary compounds are unsuitable. See Pearse's Histochemistry, Vol. 1 (3rd ed., Churchill-Livingstone, Edinburgh, 1980) page 107 for a proper account