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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃(运输条件:冰袋低温运输)
- 英文名:
UTP
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
63-39-8
- 规格:
1ml
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文献和实验HEPES sodium salt10mM EDTApH buffer to 7.8For 500mL 10 X FF: add 23.8g HEPES and 1.9g EDTA to 500mL with water (don’t forget to pH to 7.8)________________________________________________________________________To prepare RNA for loading:I usually load
Hybridisation to RNA in Tissue Sections
of 3M NaAcetate pH 5.3 and 1ml of absolute ethanol. Spin for at least 15 minutes at 13,000g Wash in 70% ethanol and spin for a further 5 minutes. Carefully take off the supernatant and dry in the speedivac. Resuspend in sterile water
Making RNA probes for in situ hybridization
2 μl 10 mM GTP 100mM 2 μl 10 mM UTP 100mM 1.3 μl 6.5 mM DNP-11-UTP 10mM 7.0 μl 3.5 mM In vitro
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