Tissue/cell: Human laryngeal squamous cell carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-P21/CDKN1A Polyclonal Antibody, Unconjugated(bs-0741R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-0741R
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
H2AX (Ubiquityl Lys119) Rabbit pAb, PE-Cy7 conjugated(bs-8571R-PE-Cy7)-100ul
￥2980
TROP2 Rabbit pAb(bs-6198R)-50ul/100ul/200ul
￥1180progesterone receptor Rabbit pAb(bs-0111R)-50ul/100ul/200ul
￥1180caspase-9 p10 Rabbit pAb(bs-8502R)-50ul/100ul/200ul
￥1180P63 Mouse mAb, APC-Cy7 conjugated(bsm-33417M-APC-Cy7)-100ul
￥2980
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0741R |
| 英文名称 | CDKN1A/p21 Rabbit pAb |
| 中文名称 | p21蛋白抗体 |
| 英文别名 | CDN1A_HUMAN; Cyclin-dependent kinase inhibitor 1; CDKN1A; CAP20; CDKN1; CIP1; MDA6; MDA-6; PIC1; SDI1; WAF1; p21CIP1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6(MDA-6); |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Dog, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human P21 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 18 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Cell Cycle Inhibitors > Cip / Kip Epigenetics and Nuclear Signaling > Cell cycle > Cell Cycle Inhibitors > Cip/Kip Epigenetics and Nuclear Signaling > Chromatin Binding Proteins > DNA / RNA binding Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Co-activators/co-repressors Epigenetics and Nuclear Signaling > Transcription > Domain Families > Zinc Finger Signal Transduction > Signaling Pathway > Nuclear Signaling > Nuclear Hormone Receptors > Co-activators/co-repressors |
| 亚基 | Interacts with HDAC1; the interaction is prevented by competitive binding of C10orf90/FATS to HDAC1 facilitating acetylation and protein stabilization of CDKN1A/p21. Interacts with MKRN1. Interacts with PSMA3. Interacts with PCNA. Component of the ternary complex, cyclin D-CDK4-CDKN1A. Interacts (via its N-terminal domain) with CDK4; the interaction promotes the assembly of the cyclin D-CDK4 complex, its nuclear translocation and promotes the cyclin D-dependent enzyme activity of CDK4. Binding to CDK2 leads to CDK2/cyclin E inactivation at the G1-S phase DNA damage checkpoint, thereby arresting cells at the G1-S transition during DNA repair. Interacts with PIM1. |
| 亚细胞定位 | Cytoplasmic and Nuclear. |
| 组织特异性 | Expressed in all adult tissues, with 5-fold lower levels observed in the brain. |
| 翻译后修饰 | Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1. |
| 相似性 | Belongs to the CDI family. |
| 功能 | May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. Two families of cyclin dependent kinase inhibitors (CKIs) have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 and Cdk6 specifically. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test } |
Images provided the Independent Validation Program (badge number 029649)Formalin-fixed and paraffin embedded human testis labeled with Rabbit Anti-CDKN1A Polyclonal Antibody (bs-0741R) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.
Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CDKN1A/p21) Polyclonal Antibody, Unconjugated (bs-0741R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control: 293T.
Primary Antibody (green line): Rabbit Anti-CDKN1A/p21 antibody (bs-0741R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-CDKN1A/p21 antibody (bs-0741R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDKN1A) Polyclonal Antibody, Unconjugated (bs-0741R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDKN1A) Polyclonal Antibody, Unconjugated (bs-0741R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDKN1A) Polyclonal Antibody, Unconjugated (bs-0741R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDKN1A) Polyclonal Antibody, Unconjugated (bs-0741R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDKN1A) Polyclonal Antibody, Unconjugated (bs-0741R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDKN1A) Polyclonal Antibody, Unconjugated (bs-0741R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 7.666 }}] {Feng Cui. et al. ML216 Prevents DNA Damage-Induced Senescence by Modulating DBC1–BLM Interaction. CELLS-BASEL. 2023 Jan;12(1):145} {IHC} {Mouse}
[IF={{ 6.7 }}] {Yan Li. et al. Discovery of ganoderic acid A (GAA) PROTACs as MDM2 protein degraders for the treatment of breast cancer. EUR J MED CHEM. 2024 Apr;270:116367} {IF,WB} {Human}
[IF={{ 5.572 }}] {Yue Zhang. et al. Dietary selenium excess affected spermatogenesis via DNA damage and telomere-related cell senescence and apoptosis in mice. FOOD CHEM TOXICOL. 2023 Jan;171:113556} {WB} {Mouse}
[IF={{ 5.223 }}] {Keiichi Hiramoto. et al. Bacillus coagulans (species of lactic acid-forming Bacillus bacteria) ameliorates azoxymethane and dextran sodium sulfate-induced colon cancer in mice. J FUNCT FOODS. 2023 Jan;100:105406} {IHC} {Mouse}
[IF={{ 4.8 }}] {Junya Nishimura. et al. CDDP-induced desmoplasia-like changes in oral cancer tissues are related to SASP-related factors induced by the senescence of cancer cells. INT IMMUNOPHARMACOL. 2024 Jul;136:112377} {ICC,IHC} {Mouse}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
. Retrieved 2010-11-29. ^ Nielsen BS, Jørgensen S, Fog JU, Søkilde R, Christensen IJ, Hansen U, Brünner N, Baker A, Møller S, Nielsen HJ (October 2010). "High levels of microRNA-21 in the stroma of colorectal cancers predict short disease-free survival
+oieSKeKcgxGinlRPl7uja5iYeDdc+K3eZhXhIOHOy+p9cxrGU9WCTkVcT5iRR/BsR+N0IDPSu3x0pXo0IRAQiAhGBiEBE4ABCwPnwbu0wGmW8ULj7NRb20DLRYqFW1Up6ODBube+wknwaZ5w/y92bXLbV5K3CJ+hhzKwdSHCmVrada2Qy0eyxvi0r7Lkl99ukcaOkRe60Sj82E8qgnmxNtzHGRRyZQtf1g41GIMau5W7ITlqbiDRR0CA3tPhIsEdmP5hZ
技术资料暂无技术资料 索取技术资料
文献支持
CDKN1A/p21 Rabbit pAb(bs-0741R)-50ul/100ul/200ul
￥1180 - 2800
