Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-CEA antibody (bs-0719R)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
- ￥1180 - 2800
- Bioss
- bs-0719R
- 2025年10月24日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0719R |
| 英文名称 | CEA Rabbit pAb |
| 中文名称 | 癌胚抗原抗体 |
| 英文别名 | Carcinoembryonic antigen; Carcinoembryonic antigen related cell adhesion molecule 5; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CD66e antigen; CEACAM5; CEAM5_HUMAN; DKFZp781M2392; Meconium antigen 100. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/test Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CEA/CD66e/CEACAM5 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 150-200 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor biomarkers > Tumor antigens Cancer > Tumor immunology > Tumor-associated antigens Stem Cells > Mesenchymal Stem Cells > Surface Molecules Tags & Cell Markers > Cell Type Markers > Tumor Associated |
| 亚基 | Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. |
| 亚细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor |
| 组织特异性 | Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. |
| 翻译后修饰 | Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. |
| 相似性 | Belongs to the immunoglobulin superfamily. CEA family. |
| 功能 | Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/test} |
Blank control: MCF7.
Primary Antibody (green line): Rabbit Anti-CEA antibody (bs-0719R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-CEA antibody (bs-0719R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEA) Polyclonal Antibody, Unconjugated (bs-0719R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEA) Polyclonal Antibody, Unconjugated (bs-0719R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEA) Polyclonal Antibody, Unconjugated (bs-0719R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
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文献和实验该产品被引用文献
[IF={{ 8.008 }}] {Li Fu. et al. A General Route for Chemiluminescence of n-Type Au Nanocrystals. ANAL CHEM. 2022;94(24):8811–8817} {Other} {}
[IF={{ 6.914 }}] {Chang N et al. Low cost 3D microfluidic chips for multiplex protein detection based on photonic crystal beads. Lab Chip. 2018 Dec 7;18(23):3638-3644.} {Other} {Human}
[IF={{ 6.45 }}] {Chen, Ze-Zhong, et al. "Indirect immunofluorescence detection of E. coli O157: H7 with fluorescent silica Nanoparticles." Biosensors and Bioelectronics (2014).} {Other} {=""}
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= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
，发现某种治疗基因耙标，以及利用基因表达情况的变化观察药物和基因治疗的疗效。Soeth等在tet-off启动子系统控制下从完整结肠癌细胞抽提以CEA为耙标的核酶。这种方法可以调控50％的CEA mRNA和蛋白水平，并用cDNA基因芯片检测CEA介导的细胞基因表达谱的改变。通过273条基因的筛查发现，CEA改变不同癌相关基因组、特别是细胞周期和凋亡基因。尽管细胞周期基因表达水平的改变是双向的调节异常，但是细胞凋亡基因被CEA间接下调。在平行表型研究中，CEA不影响细胞周期和增生比例。然而，CEA显著
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CEA Rabbit pAb(bs-0719R)-50ul/100ul/200ul
￥1180 - 2800
