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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
联硕生物
- 规格:
50ML

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文献和实验ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
1 mM phenylmethylsulfonyl fluoride (PMSF)3 1 mM EDTA 0.3 M NaCl 1 mg/ml BSA 2% IGEPAL CA-630 (v/v)4 0.02% NaN3 Store at 4 C indefinitely Washing Buffer 50 mM Tris-acetate, pH 7.5 0.3 M NaCl 0.5% IGEPAL CA-630 0.1% sodium
Hepes. Harvest cells by putting 20mls of -Ca-Mg PBS, buffered with 0.02M Hepes, and 1mM EDTA on each plate for 10min in the 37 C incubator. After the 10min, collect some of the PBS in a 50ml conical tube before pipeting
Microscale Hot Borate RNA Extraction from Cotton Tissue--从棉花组织中提取微量RNA
*1% IGEPAL CA-630 (Nonidet P-40, NP-40) 1.0 mL*2% (w/v) PVP-40 2.0 grams* = add
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