Direct Red 23,3441-14-3，212490

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

A[hygro] was co-transformed with the pSC101BADγβαA[hygro] vector into HS996 electrocompetent E. coli cells harbouring the pSC101BADγβαA[tet] vector and recombination was achieved using standard Red/ET protocols (8) and selection with Ap 50 μg/ml

Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion Affinity Media and Direct Analysis by MALDI-TOF-MS

peptides is also described.MATERIALSReagentsAcetic acid (100 mM)Ammonium bicarbonate (50 mM, pH 8.0)Calf intestinal alkaline phosphataseCarboxypeptidase Y (1 µg/µL in H2O)EDTA (100 mM in H2O, pH 8.0)Ferric chloride (FeCl3) (100 mM)Gallium chloride (GaCl

Direct Immunofluorescence Labeling

Description This is the method for direct immunofluorescence labeling; that is, the antibodies have the fluorescent dye attached. Direct labeling is simpler and quicker than indirect labeling. Procedure Adjust the cell concentration