Sample:
_x000D_
MDCK(Dog) Cell Lysate at 40 ug
_x000D_
Primary: Anti-Nephrin (bs-10233R) at 1/500 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 138 kD
_x000D_
Observed band size: 138 kD
- ￥1180 - 2800
- Bioss
- bs-10233R
- 2026年10月17日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10233R |
| 英文名称 | Nephrin Rabbit pAb |
| 中文名称 | 肾小球细胞粘附分子受体抗体 |
| 英文别名 | CNF; NPHN; Nephrosis 1 congenital Finnish type; NPHS 1; NPHS1; Renal glomerulus specific cell adhesion receptor; Renal glomerulus-specific cell adhesion receptor; NPHN_HUMAN . |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat, Dog (Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Nephrin |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 138 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Cell Adhesion Molecules > Endothelial |
| 亚基 | Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL/NEPH1 and KIRREL2; the interaction with KIRREL is dependent on KIRREL glycosylation. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1 (By similarity). Interacts with NPHS2. |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein (Potential). Note=Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane. |
| 组织特异性 | Specifically expressed in podocytes of kidney glomeruli. Expressed in kidney glomeruli. In the embryo,expressed in the mesonephric kidney at E11 with strong expression in cranial tubules with podocyte-like structures. Expression is observed in the podocytes of the developing kidney from E13. High expression is also detected in the developing cerebellum, hindbrain, spinal cord, retina and hypothalamus. Expressed in skeletal muscle during myoblast fusion such as in the adult following acute injury and in the embryo but not detected in uninjured adult skeletal muscle. Isoform 1 and isoform 2 are expressed in the newborn brain and developing cerebellum. Isoform 1 is the predominant isoform in adult kidney. |
| 翻译后修饰 | Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1._x000D_ DISEASE : Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. |
| 相似性 | Belongs to the immunoglobulin superfamily. Contains 1 fibronectin type-III domain. Contains 8 Ig-like C2-type (immunoglobulin-like) domains. |
| 功能 | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Sample:
_x000D_ Liver (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-Nephrin (bs-10233R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 138 kD
_x000D_ Observed band size: 138 kD
_x000D_
_x000D_ Liver (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-Nephrin (bs-10233R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 138 kD
_x000D_ Observed band size: 138 kD
_x000D_
Blank control:293T.
_x000D_ Primary Antibody (green line): Rabbit Anti-Nephrin antibody (bs-10233R)
_x000D_ Dilution: 1ug/Test;
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-FITC
_x000D_ Dilution: 0.5ug/Test.
_x000D_ Protocol
_x000D_ The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
_x000D_ Primary Antibody (green line): Rabbit Anti-Nephrin antibody (bs-10233R)
_x000D_ Dilution: 1ug/Test;
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-FITC
_x000D_ Dilution: 0.5ug/Test.
_x000D_ Protocol
_x000D_ The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-Nephrin Polyclonal Antibody, Unconjugated(bs-10233R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-Nephrin Polyclonal Antibody, Unconjugated(bs-10233R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
the cells(293T) were fixed with 2% Paraformaldehyde (10 min).
_x000D_ Isotype Control Antibody: Rabbit IgG(orange) ; _x000D_ Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), _x000D_ Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; _x000D_ Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
_x000D_ Isotype Control Antibody: Rabbit IgG(orange) ; _x000D_ Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), _x000D_ Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; _x000D_ Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
Blank control: RSC96(blue), the cells were fixed with 2% Paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
_x000D_ Isotype Control Antibody: Rabbit IgG(orange) ; _x000D_ Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), _x000D_ Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; _x000D_ Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green)._x000D_
_x000D_ Isotype Control Antibody: Rabbit IgG(orange) ; _x000D_ Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), _x000D_ Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; _x000D_ Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green)._x000D_
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NPHS1 | Nephrin Rabbit pAb(bs-10233R)-50ul/100ul/200ul
￥1180 - 2800
