Blank control: SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-CD24 antibody (bs-4891R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-4891R
- 2025年10月16日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4891R |
| 英文名称 | CD24 Rabbit pAb |
| 中文名称 | CD24抗体 |
| 英文别名 | CD 24; CD24 antigen(small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24A; GPI linked surface mucin; Heat stable antigen; HSA; Nectadrin; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; Cd24a; Ly-52; CD24_HUMAN. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse CD24 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 8 kDa |
| 实际分子量 | 30-45kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Cellular metabolic process Cancer > Cancer Metabolism > Response to hypoxia Cancer > Tumor biomarkers > Tumor antigens Immunology > Adaptive Immunity > B Cells > CD Metabolism > Pathways and Processes > Metabolism processes > Hypoxia Neuroscience > Cell Type Marker > Neural Stem Cell marker Stem Cells > Hematopoietic Progenitors > Hematopoietic Stem Cells > Human Lineage Negative Stem Cells > Hematopoietic Progenitors > Lymphoid > B Lymphocytic Lineage Stem Cells > Hematopoietic Progenitors > Lymphoid > T Lymphocytic Lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Dendritic Cell Lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Erythrocytic Lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Monocytic Lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Neutrophil Lineage |
| 亚细胞定位 | Cell membrane. |
| 组织特异性 | B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells. |
| 翻译后修饰 | Extensively O-glycosylated. |
| 相似性 | Belongs to the CD24 family. |
| 功能 | Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1ug/Test} |
Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Bone tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Mouse Cerebrum tissue lysates
Primary: Anti-CD24 (bs-4891R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 8 kD
Observed band size: 33 kD
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Bone tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Mouse Cerebrum tissue lysates
Primary: Anti-CD24 (bs-4891R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 8 kD
Observed band size: 33 kD
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CD24 Rabbit pAb(bs-4891R)-50ul/100ul/200ul
￥1180 - 2800
