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His tag Mouse mAb(bsm-33004M)-

100ul/500ul/200ul/1000ul
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  • ¥580 - 4680
  • Bioss已认证
  • bsm-33004M
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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    • 规格

      100ul/500ul/200ul/1000ul

    规格:100ul产品价格:¥580.0
    规格:500ul产品价格:¥2280.0
    规格:200ul产品价格:¥980.0
    规格:1000ul产品价格:¥4680.0
    产品编号bsm-33004M
    英文名称His tag Mouse mAb
    中文名称聚组氨酸抗体标签单克隆抗体
    英文别名HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
    产品应用WB=1:5000-50000, ELISA=1:1000-5000

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Species independent
    抗体来源Mouse
    免疫原KLH conjugated HHHHHH peptide
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein G
    克隆类型Monoclonal
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Signal Transduction > Antibodies > 6x his tag®

    Tags & Cell Markers > Epitope Tags > His Tag

    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

     

    应用推荐稀释比例
    {WB}{1:5000-50000}
    {ELISA}{1:1000-5000}

     

    产品细节图片1
    50 ng Recombinant GFP protein (bs-33009P) per lane probed with His tag monoclonal antibody respectively, unconjugated (bsm-33004M) at 1:5000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 9.7 }}] {Qian He. et al. Designing a reengineered probiotic yeast to spontaneously degrade residual antibiotics in gut during antimicrobial therapy. J CLEAN PROD. 2024 Nov;:144177} {WB} {Saccharomyces boulardii}

    [IF={{ 8.2 }}] {Beibei Wang. et al. Identification and functional analysis of Toll-like receptor 2 from razor clam Sinonovacula constricta. INT J BIOL MACROMOL. 2024 Apr;265:131029} {ELISA} {Mouse}

    [IF={{ 7.7 }}] {Haoge Hu. et al.Molecular characterization and immunological functions of the antimicrobial peptide theromacin from razor clam Sinonovacula constricta..International Journal of Biological Macromolecules.2025 Mar 24;308(Pt 2):142312.} {Elisa} {Sinonovacula constricta}

    [IF={{ 4.961 }}] {Rong Yue. et al. Study of the Effects of Several SARS-CoV-2 Structural Proteins on Antiviral Immunity. VACCINES-BASEL. 2023 Mar;11(3):524} {WB} {Human}

    [IF={{ 4.6 }}] {Mengqi Xie. et al.The Mycoplasma hyopneumoniae protein Mhp274 elicits mucosal and systemic immune responses in mice.FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY.2025 Feb 7:15:1516944.} {Western blot} {}

    相关实验
    • Dual- and Triple-Color Fluorospot

      in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb

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    文献支持
    His tag Mouse mAb(bsm-33004M)-100ul/500ul/200ul/1000ul
    ¥580 - 4680