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LY75 Recombinant Rabbit mAb(bs

m-60593R)-50ul/100ul/25ul
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  • ￥800 - 2500
  • Bioss已认证
  • bsm-60593R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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    • 规格：

      50ul/100ul/25ul

    规格：50ul产品价格：￥1400.0
    规格：100ul产品价格：￥2500.0
    规格：25ul产品价格：￥800.0
    产品编号bsm-60593R
    英文名称LY75 Recombinant Rabbit mAb
    中文名称淋巴细胞抗原75（CD205）重组兔单抗
    英文别名LY75_HUMAN; Lymphocyte antigen 75; CD205; CLEC13B; Ly-75; C-type lectin domain family 13 member B; DEC-205; gp200-MR6;
    产品应用IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, WB=1:500-2000

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat
    抗体来源Rabbit
    免疫原A synthesized peptide derived from human DEC 205
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Recombinant
    理论分子量195 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Immunology > Adaptive Immunity > B Cells > Non-CD

    Tags & Cell Markers > Cell Type Markers > Tumor Associated

    亚细胞定位Membrane; Single-pass type I membrane protein.
    组织特异性Expressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B-lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells.
    翻译后修饰N-glycosylated.
    相似性Contains 10 C-type lectin domains.
    Contains 1 fibronectin type-II domain.
    Contains 1 ricin B-type lectin domain.
    功能Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment. Causes reduced proliferation of B-lymphocytes.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Predicted to enable signaling receptor activity. Predicted to be involved in immune response and inflammatory response. Located in extracellular exosome. [provided by Alliance of Genome Resources, Apr 2022]

     

    应用推荐稀释比例
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {WB}{1:500-2000}

     

    产品细节图片1
    25 ug total protein per lane of various lysates (see on figure) probed with LY75 monoclonal antibody, unconjugated (bsm-60593R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LY75 Monoclonal Antibody, Unconjugated(bsm-60593R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LY75 Monoclonal Antibody, Unconjugated(bsm-60593R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LY75 Monoclonal Antibody, Unconjugated(bsm-60593R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LY75 Monoclonal Antibody, Unconjugated(bsm-60593R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片6
    P‌‌araformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LY75 Monoclonal Antibody, Unconjugated(bsm-60593R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

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