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| 产品编号 | bs-17686P |
| 英文名称 | SPRED2 Antibody Blocking Peptide |
| 中文名称 | SPRED2蛋白封闭多肽 |
| 英文别名 | C79158; FLJ21897; FLJ31917; SPRE2_HUMAN; MGC163164; Spred 2; Sprouty protein with EVH 1 domain 2 related sequence; sprouty related EVH1 domain containing 2; Sprouty related protein with EVH 1 domain 2; zgc:77284. |
| 纯化方法 | HPLC |
| 研究领域 | Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > MAPK Pathway Signal Transduction > Signaling Pathway > G Protein Signaling > Small G Proteins > Ras Family |
| 亚基 | Interacts with Ras (By similarity). Homodimer and heterodimer. Able to interact with SPRED1 to form heterodimers. |
| 亚细胞定位 | Cell Membrane - peripheral membrane protein and Cytoplasmic. |
| 组织特异性 | Expressed in liver, skin, small intestine, salivary gland and prostate. |
| 翻译后修饰 | Phosphorylated on tyrosine. Phosphorylation of Tyr-228 and Tyr-231 are required for ubiquitination. Ubiquitinated; leading to degradation by the proteasome._x000D_ |
| 相似性 | Contains 1 KBD domain. Contains 1 SPR (sprouty) domain. Contains 1 WH1 domain._x000D_ |
| 功能 | Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008] |
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文献和实验Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
, activate AEC buffer using H2 O2 (see Step 25). Anti-CD3 monoclonal antibody (mAb) (Fisher Scientific) Blocking buffer for ELISPOT assay CEF peptide pool (available from the NIH AIDS Research and Reagent Program and other commercial
0.3% hydrogen peroxide (prepare immediately before use) Blocking buffer (see recipe ) HCV‐specific primary antibody (Ab), e.g
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