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| 产品编号 | bs-4606PB |
| 英文名称 | Gibberellins/BSA |
| 中文名称 | 赤霉素牛血清白蛋白偶联物 |
| 英文别名 | free gibberellin; conjugated gibberellin. |
| 性状 | Liquid |
| 储存液 | 0.01M TBS(pH7.4). |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | There are two classes of gibberellins: the 19-carbon gibberellins and the 20-carbon gibberellins. The 19-carbon gibberellins,formed from 20-carbon gibberellins,are the bio-logically active forms. Gibberellins also vary according to the position and number of hydroxyl groups linked to the carbon atoms of the ent-gibberellane skeleton. Hydroxylation has a profound influence on biological activity. Probably the best-defined role for gibberellins in regulating the developmental processes in higher plants is stem growth. The cellular basis for gibberellin-induced stem growth can be either an increase in the length of pith cells in the stem or primarily the production of a greater number of cells. Applied gibberellins can often promote germination of dormant seeds,a capability suggesting that gibberellins are involved in the process of breaking dormancy. Gibberellins are intimately involved in other aspects of seed germination as well. Applied gibberellins promote or induce flowering in plants that require either cold or long days for flower induction. Gibberellin is probably not the flowering hormone or floral stimulus,because the floral stimulus appears to be identical or similar in all response types. The application of gibberellins often modifies sex expression,usually causing an increase in the number of male flowers. See also Dormancy; Flower; Plant growth; Seed. Although gibberellins have limited use in agriculture compared with other agricultural chemicals such as herbicides,several important applications have been developed,including the production of seedless grapes. Application of gibberellin at bloom results in increased berry size and reduced berry rotting. Gibberellins are also used to increase barley malt yields for brewing and to reduce the time necessary for the malting process to reach completion. Gibberellins have found significant applications in plant breeding. Other uses for gibberellin in agriculture include reduction of rind discoloration in citrus fruits,increased yield in sugarcane,stimulation of fruit set in fruit trees,and increased petiole growth in celery. See also Plant hormones |
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文献和实验[IF={{ 8.5 }}] {Lingqin Meng. et al. Highly sensitive antibiotic sensing based on optical weak value amplification: A case study of chloramphenicol. FOOD CHEM. 2024 Jun;:140184} {Other} {}
Maxisorb 442404)Micro test tubes, 大头针或喷火枪药品试剂:(a) Buffer A-0(b) BSA标准品 (Bio-Rad, bovine serum albumin, 100 μg/mL)(c) 未知浓度样本 (unknown BSA, 0.8 mL, 由教师调配发给)(d) Dye Reagent (Bio-Rad 500-0006) 先以水稀释四倍备用。 以染料Coomassie brilliant blue G-250 配制的溶液,勿与胶体电泳染色用的CBR-250
除>95%的蛋白提取和样品制备过程中所使用的去垢剂,而蛋白和多肽损失极少。此树脂适用于常用去垢剂的去除,如浓度在0.5-1%的SDS、Triton X-100、NP-40和CHAPS。目前有预装的离心柱和离心板形式,最多可处理100 µL样品。另外,您也可以购买HiPPR Detergent Removal Spin Column Kit,其中包含树脂浆和空的离心柱,便于您自己调整样品体积。 整个处理过程相当简单,仅需15分钟。首先是离心去除保存液,之后用缓冲液平衡树脂并离心,重复
Bromodeoxyuridine Staining of Mammary Tissue
oC in a humid box. 3. Rinse 2 x 3 min in PBS. 4. Treat sections with 0.3% hydrogen peroxide for 10 minutes at room temperature. 5. Rinse 2 x 3 min in PBS. 6. Treat with 2N HCl for 1 hour at 37o C. 7. Rinse 2 x 5 min in 0.1M borate buffer pH
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