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- 应用范围:
产品信息以Bioss网站为准
- 规格:
25T/50T/100T
| 规格: | 25T | 产品价格: | ¥580.0 |
|---|---|---|---|
| 规格: | 50T | 产品价格: | ¥980.0 |
| 规格: | 100T | 产品价格: | ¥1380.0 |
| 产品编号 | bsm-30128M-PE |
| 英文名称 | human CD80 Mouse mAb, PE conjugated |
| 中文名称 | PE标记人CD80单克隆抗体 |
| 英文别名 | Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80 antigen(CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_HUMAN; Costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80. |
| 产品应用 | Flow-Cyt=10ul/Test Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 亚型 | Mouse IgG1, k |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 32 kDa |
| 实际分子量 | 60kDa |
| 储存液 | 0.01M PBS (pH7.4), 0.5% BSA, 0.02% Proclin300. |
| 研究领域 | Immunology > Adaptive Immunity > B Cells > CD Stem Cells > Hematopoietic Progenitors > Lymphoid > B Lymphocytic Lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Dendritic Cell Lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Monocytic Lineage |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Expressed on activated B-cells, macrophages and dendritic cells. |
| 相似性 | Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| 功能 | Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. |
| 保存条件 | Store at 2-8℃. Protect from light. Avoid freezing. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {10ul/Test} |
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
3-PerCP-Cy5.5 PCP55-30004 CD4-FITC FITC-30005 IFN-γ-PE PE-30053 IL4-APC APC-30043 Mouse Th1/Th2 细胞群检测 CD3-PerCP-Cy5.5 PCP55-30002 CD4-FITC FITC-30128 IFN-γ-PE PE-30074 IL4-APC APC-30026 Human Treg细胞群检测 CD4-FITC FITC-30005 CD25-PE PE-30035
FITC-30128 IFN-γ-PE PE-30074 IL4-APC APC-30026 Human Treg细胞群检测 CD4-FITC FITC-30005 CD25-PE PE-30035 CD3-PerCP-Cy5.5 PCP55-30004 CD127-FineTest®647 F647-30033 Mouse Treg细胞群检测 CD4-FITC FITC-30128 CD
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