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- bsm-30049M-PE
- 2025年10月15日
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产品信息以Bioss网站为准
- 规格：
25T/50T/100T
| 规格： | 25T | 产品价格： | ￥680.0 |
|---|---|---|---|
| 规格： | 50T | 产品价格： | ￥1080.0 |
| 规格： | 100T | 产品价格： | ￥1680.0 |
| 产品编号 | bsm-30049M-PE |
| 英文名称 | human CD62L Mouse mAb, PE conjugated |
| 中文名称 | PE标记人CD62L单克隆抗体 |
| 英文别名 | L Selectin; A.11; AI528707; CD62 antigen ligand; CD62 antigen-like family member L; gp90 MEL; hLHRc; IgA nephropathy, susceptibility to, included; L Selectin; LAM1; LECAM1; LEU8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LSEL; Ly-22; Ly-m22; Lyam-1; LYAM1; Lymph node homing receptor; Lymphocyte adhesion molecule 1; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen; PLNHR; Selectin L; Selectin, lymphocyte; SELL; TQ1; LYAM1_HUMAN. |
| 产品应用 | Flow-Cyt=10ul/Test Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 亚型 | Mouse IgG1, k |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 88 kDa |
| 实际分子量 | 140kDa |
| 储存液 | 0.01M PBS (pH7.4), 0.5% BSA, 0.02% Proclin300. |
| 研究领域 | Cardiovascular > Blood Immunology > Adaptive Immunity > Regulatory T Cells Immunology > Adaptive Immunity > T Cells > CD Stem Cells > Hematopoietic Progenitors > Lymphoid > T Lymphocytic Lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Neutrophil Lineage |
| 亚基 | Interaction with PSGL1/SELPLG and PODXL2 is required for promoting recruitment and rolling of leukocytes. This interaction is dependent on the sialyl Lewis X glycan modification of PSGL1 and PODXL2, and tyrosine sulfation modifications of PSGL1. Sulfation on 'Tyr-51' of PSGL1 is important for L-selectin binding. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Expressed in B-cell lines and T-lymphocytes. |
| 相似性 | Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain. Contains 1 EGF-like domain. Contains 2 Sushi (CCP/SCR) domains. |
| 功能 | Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. |
| 保存条件 | Store at 2-8℃. Protect from light. Avoid freezing. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {10ul/Test} |
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
Immunofluorescent Staining of Mouse and Rat Leukocytes
, or fluorochrome-conjugated mAbs) to predetermined optimal concentrations (see Staining Tips) in wash buffer and deliver to the wells of a U-bottom microtiter plate in a volume of 50 µl. Deliver 106 cells in 50 µl to each well already containing 50 µl of mAb
Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
the same antigen is the antibody TG343 (Millipore, cat. no. MAB4346) PE rat anti‐mouse CD 90.2 (0.2 mg/ml; BD Pharmingen, cat. no. 553014)
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