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- bsm-33346M-HRP
- 2025年10月15日
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100ul
| 产品编号 | bsm-33346M-HRP |
| 英文名称 | Flag Tag Mouse mAb, HRP conjugated |
| 中文名称 | HRP标记的Flag Tag标签单克隆抗体 |
| 英文别名 | DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; DYKDDDDK-Tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG-tag. |
| 产品应用 | WB=1:5000-50000, ELISA=1:5000-10000 Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 免疫原 | KLH conjugated Flag Tag |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Tags & Cell Markers > Epitope Tags > DDDDK Tag |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This is a useful tool for the localisation and characterisation of DDDDK tagged proteins. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:5000-50000} |
| {ELISA} | {1:5000-10000} |
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文献和实验. For alkaline phosphatase, use a 1:3000 dilution of Biorad goat anti-mouse or anti-rabbit alkaline phosphatase conjugated antibody in blotto. Incubate on rocker 1 hr at 25℃. 8. Wash 3X3min in TBS/Tween. 9. Alkaline phosphatase development: Add developing
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
) HRP‐conjugated anti‐flag antibody Tyramide signal amplification (TSA) kit (Life Technologies, cat. no. T30953) containing
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