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- Bioss
- bs-3680R-Bio
- 2025年10月15日
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100ul
| 产品编号 | bs-3680R-Bio |
| 英文名称 | APC Rabbit pAb, Biotin conjugated |
| 中文名称 | Biotin标记的腺瘤样息肉抗体 |
| 英文别名 | Adenomatous Polyposis Coli; Adenomatous polyposis coli protein; APC_HUMAN; CC1; Deleted in polyposis 2.5; DP2; DP2.5; DP3; FAP; FPC; GS; Protein APC. |
| 中文别名 | 腺瘤性结肠息肉病蛋白； |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human APC |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell cycle > Cell division Cancer > Oncoproteins/suppressors > Tumor suppressors > PTC & Wnt pathway Cardiovascular > Heart > Cardiogenesis > Transcription factors/regulators Cell Biology > Cell Cycle > Cell Division > Spindle Epigenetics and Nuclear Signaling > Transcription > Cancer susceptibility > Tumor Suppressors Neuroscience > Cell Type Marker > Glia marker > Oligodendrocyte marker Stem Cells > Signaling Pathways > Wnt > Cytoplasmic |
| 亚基 | Forms homooligomers and heterooligomers with APC2. Interacts with DIAPH1 and DIAPH2. Interacts withPDZ domains of DLG1 and DLG3. Associates with catenins. Binds axin. Interacts with ARHGEF4 (via N-terminus). Interacts with MAPRE1 (viaC-terminus); probably required for APC targeting to the growingmicrotubule plus ends. Interacts with MAPRE2 and MAPRE3 (viaC-terminus). Found in a complex consisting of ARHGEF4, APC andCTNNB1. Interacts with SCRIB; may mediate APC targeting to adherensjunctions of epithelial cells. Interacts with SPATA13 (viaN-terminus and SH3 domain). Interacts with ASAP1 (via SH3 domain). Found in a complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B. Interacts at the cell membrane with FAM123A and FAM123B (via ARM repeats). |
| 亚细胞定位 | Cell junction, adherens junction. Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle membrane. Cytoplasm. Cell membrane. Note=Associated with the microtubule network at the growing distal tip of microtubules. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. |
| 组织特异性 | Expressed in a variety of tissues. |
| 翻译后修饰 | Phosphorylated by GSK3B.
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID. |
| 相似性 | Belongs to the adenomatous polyposis coli (APC) family. Contains 7 ARM repeats. |
| 功能 | Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验- or triple-labeling immunofluorescence, it is necessary to use primary antibodies raised in different host species such as mouse, rabbit, and goat. However, in many cases, suitable primary antibodies raised in different species are unavailable
: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation
dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA
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