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- bs-13771R-FITC
- 2026年10月17日
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100ul
| 产品编号 | bs-13771R-FITC |
| 英文名称 | ITGA11 Rabbit pAb, FITC conjugated |
| 中文名称 | FITC标记的整合素α11抗体 |
| 英文别名 | integrin a11; Integrin alpha 11; Integrin alpha-11; ITA11_HUMAN; Integrin α11. |
| 产品应用 | IF=1:100-500, ICC/IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ITGA11 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Invasion/microenvironment > ECM > Cell adhesion > Integrins Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Integrins > Alpha |
| 亚细胞定位 | Membrane. |
| 组织特异性 | According to PubMed:10464311, highest levels of expression in uterus and heart, intermediate levels in skeletal muscle and intermediate to low levels in pancreas, kidney and placenta. According to PubMed:10486209, also found in brain, colon, lung, small intestine, stomach, testis, salivary glands, thyroid glands and prostate. Very low levels in peripheral blood lymphocytes, fetal brain and fetal liver. |
| 相似性 | Belongs to the integrin alpha chain family. _x000D_ Contains 7 FG-GAP repeats. _x000D_ Contains 1 VWFA domain. |
| 功能 | Integrin alpha-11/beta-1 is a receptor for collagen. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Integrins are heterodimers composed of noncovalently associated transmembrane alpha and beta subunits, which heterodimerize to produce more than 20 different receptors. Most Integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, collagen (COL) and Vitronectin. Certain Integrins can also bind to soluble ligands, such as Fibrinogen, or to counterreceptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), leading to cellular aggregation. Integrin ?1, also known as ITGA11 or MSTP018, is a 1,189 amino acid single-pass type I membrane protein that contains one VWFA domain and seven FG-GAP repeats. Expressed at high levels in heart and uterus and present at lower levels in pancreas, kidney, skeletal muscle, placenta, lung, colon and brain, Integrin alpha 11 exists as a heterodimer with Integrin beta1 and functions as a receptor for collagen. |
| 应用 | 推荐稀释比例 |
| {IF} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
reagent; BD Biosciences 555899) (for Protocol 4) Buffer for cell staining and cell sorting (for Protocol 4; see Step 72) Complement, reconstituted from rabbit (Cedarlane CL3331) (for Protocol 3) ESC medium (for Protocols 1, 2) Freezing
rabbit serum albumin 兔血清白蛋白 R-Sc Reed-Sternberg's cell 瑞-司氏细胞 Z/R Reinheit Zahl 光密度比值 SAV small agranular vesicle 无颗粒小泡 SBA soybean agglutinin 大豆凝集素 SC secretory component 分泌成份 SCV small clear vesicle 清亮小泡 SDS sodium
变性，需在4℃进行，同时用pH8.0缓冲液稀释抗血清，以减少蛋白浓度过高而发生共沉淀。铵盐的溶解度不随温度变化而明显改变，0℃和25℃仅差3%，而钠盐则相差5倍，因此常在低温沉淀时用铵盐，室温沉淀用钠盐。铵盐对抗体的标记反应（如FITC和biotin标记时）有一定的干扰作用。 盐析法只能部分纯化抗体，更高纯度的抗体制剂可用层析法制备。IgM五聚体相对分子质量达9.7×10，比血清中任何其他蛋白都大，用分子筛层析很容易将其纯化。IgG在PH8.0时带负电荷，能与DEAE纤维素上的阳离子结合，因此可用
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