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- 2025年10月15日
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| 规格： | 10ug | 产品价格： | ￥150.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ￥850.0 |
| 规格： | 500ug | 产品价格： | ￥3400.0 |
| 产品编号 | bs-48090P |
| 英文名称 | Recombinant human Vitronectin protein (Active, CHO) |
| 中文名称 | 重组人玻连蛋白 |
| 英文别名 | VN; S-Protein; Serum-Spreading Factor; V75; VTN; |
| 性状 | Lyophilized |
| 纯化方法 | AC |
| 理论分子量 | 52.3 |
| 储存液 | Lyophilized from a 0.22μm-filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH7.4 |
| 研究领域 | Cardiovascular > Blood > Serum Proteins Immunology > Innate Immunity > Complement > Regulatory |
| 亚基 | Monomer. Interacts with SERPINE1/PAI1 (By similarity). |
| 亚细胞定位 | Secreted, extracellular space. |
| 组织特异性 | Plasma. |
| 翻译后修饰 | Sulfated on 2 tyrosine residues (By similarity). N- and O-glycosylated (By similarity). It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both. |
| 相似性 | Contains 4 hemopexin-like domains.
Contains 1 SMB (somatomedin-B) domain. |
| 功能 | Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway (By similarity). |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 36 months at -20℃ to -80℃ in lyophilized state. 6 months at -20℃ to -80℃ under sterile conditions after reconstitution. 7-10 days at 2℃ to 8℃ under sterile conditions after reconstitution. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Vitronectin (also known as serum spreading factor, S protein of complement or epibolin) is one of the major multifunctional cell adhesive glycoproteins in mammalian plasma and serum. It is a monomeric acidic glycoprotein detected as a mixture of 75 kD and 65 kD polypeptides. Vitronectin binds to heparin, collagen, streptococci and variety of cultured cells. It also acts as an inhibitor of the complement cascade by binding to the C5b9 complex. Vitronectin promotes cell adhesion and spreading by binding through its cell attachment tripeptide Arg-Gly-Asp (RG-D), activity which is mediated by several different integrin receptors. Apart from the significance for identifying the molecule in the above situations, it also plays an important role in events such as embryonal development, deposition of vitronectin in a number of fibrotic disease states, carcinomas and metastases. |
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文献和实验deposit: NoPrice_code: AAvailability in cell line catalogues: ATCC CCL 61; IZSBS BS CL15; DSMZ ACC 110;Bibliographic references:J Exp Med 1958;108:945 Proc Nat Acad Sci USA 1968;60:1275CHO-K1 (hamster, Chinese, ovary)Morphology: epithelial-likeSpecies
后置-20℃保存 20×SSC 10×PBS （DEPC处理的）含50%甲酰胺的杂交液（HS）--- 1ml分装后置-20℃保存 10×阻断液（10×BS） Buffer I Buffer II Buffer III 探针（P） 抗Dig-Ap NBT/BCIP 二、操作流程: 1） 脱蜡至水： 二甲苯5min×2 → 90%乙醇5min → DEPC水洗涤5min×2 2） 杂交前处理： 1×PBS洗涤5min → 蛋白酶K（终浓度20ug/ml in PBS）37℃
in Renal Cancer (p 73-96) Rosamonde E. Banks, Peter J. Selby Chapter 6: Heat Shock Protein 27 in Cancer (p 97-109) Dr. Cecilia Sarto, Dr. Fulvio Magni, BS. Cristina Valsecchi, Prof. Dr. Paolo Mocarelli Chapter 7: Proteomic Approaches for Biomarker
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