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| 规格: | 10ug | 产品价格: | ¥750.0 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥2250.0 |
| 规格: | 500ug | 产品价格: | ¥9000.0 |
| 产品编号 | bs-48075P |
| 英文名称 | Recombinant human LIF protein (Active, CHO) |
| 中文名称 | 重组人白血病抑制因子 |
| 英文别名 | Leukemia Inhibitory Factor; Differentiation-Stimulating Factor; D Factor; Melanoma-Derived LPL Inhibitor; MLPLI; Emfilermin; HILDA; |
| 性状 | Lyophilized |
| 纯化方法 | AC |
| 理论分子量 | 19.7 |
| 储存液 | Lyophilized from 0.22μm-filtered solution in PBS, 5% mannital, 0.01% Tween 80, pH7.4 |
| 研究领域 | Cancer > Cell cycle > Cell differentiation Cell Biology > Cell Cycle > Cell differentiation Epigenetics and Nuclear Signaling > Transcription > Cancer susceptibility > Tumor Suppressors Immunology > Innate Immunity > Cytokines Immunology > Innate Immunity > Cytokines > IL6 Related Neuroscience > Neurology process > Neurogenesis |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the LIF/OSM family. |
| 功能 | LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 36 months at -20℃ to -80℃ in lyophilized state. 6 months at -20℃ to -80℃ under sterile conditions after reconstitution. 7-10 days at 2℃ to 8℃ under sterile conditions after reconstitution. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The leukemia inhibitory factor- LIF is a kind of cell factor that has various living creature functions.It has the leukemia cell of inducement to divide, regulating the growth metabolism of the bone organization, maintaining the basic characteristic of the Embryonicstem celles and promoting the mankind build the blood stem cell to divide to propagate, promote the cholinergic neuron to divide, obstruct the fat cell to synthesize, stimulate the muscle cell growth, regulate the liver cell to expect to respond the egg white to synthesize etc. in the acute the biology activity etc. function. |

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文献和实验deposit: NoPrice_code: AAvailability in cell line catalogues: ATCC CCL 61; IZSBS BS CL15; DSMZ ACC 110;Bibliographic references:J Exp Med 1958;108:945 Proc Nat Acad Sci USA 1968;60:1275CHO-K1 (hamster, Chinese, ovary)Morphology: epithelial-likeSpecies
后置-20℃保存 20×SSC 10×PBS (DEPC处理的)含50%甲酰胺的杂交液(HS)--- 1ml分装后置-20℃保存 10×阻断液(10×BS) Buffer I Buffer II Buffer III 探针(P) 抗Dig-Ap NBT/BCIP 二、操作流程: 1) 脱蜡至水: 二甲苯5min×2 → 90%乙醇5min → DEPC水洗涤5min×2 2) 杂交前处理: 1×PBS洗涤5min → 蛋白酶K(终浓度20ug/ml in PBS)37℃
in Renal Cancer (p 73-96) Rosamonde E. Banks, Peter J. Selby Chapter 6: Heat Shock Protein 27 in Cancer (p 97-109) Dr. Cecilia Sarto, Dr. Fulvio Magni, BS. Cristina Valsecchi, Prof. Dr. Paolo Mocarelli Chapter 7: Proteomic Approaches for Biomarker
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