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| 规格： | 10ug | 产品价格： | ￥600.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ￥1600.0 |
| 规格： | 500ug | 产品价格： | ￥6400.0 |
| 产品编号 | bs-48074P |
| 英文名称 | Recombinant human CNTF protein (Active, E.coli) |
| 中文名称 | 重组人睫状神经营养因子 |
| 英文别名 | Ciliary neurotrophic factor; |
| 性状 | Lyophilized |
| 纯化方法 | AC |
| 理论分子量 | 22.9 |
| 储存液 | Lyophilized from a 0.22μm-filtered solution containing 10mM PB, 5% Trehalose and 0.01% Tween 80, pH7.4 |
| 研究领域 | Neuroscience > Cell Type Marker > Neural Stem Cell marker Neuroscience > Neurology process > Growth and Development > Neurotrophins Stem Cells > Neural Stem Cells > Glial Restricted Lineage > Glial Progenitors |
| 亚基 | Belongs to the CNTF family. |
| 亚细胞定位 | Cytoplasm. |
| 组织特异性 | Nervous system. |
| 相似性 | Belongs to the CNTF family. |
| 功能 | CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 36 months at -20℃ to -80℃ in lyophilized state. 6 months at -20℃ to -80℃ under sterile conditions after reconstitution. 7-10 days at 2℃ to 8℃ under sterile conditions after reconstitution. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition to the predominant monocistronic transcript originating from this locus, the gene is also transcribed as a readthrough transcript with the upstream Zfp91 gene. The resulting protein is an isoform of Zfp91 and does not include Cntf sequence. Readthrough transcription of Zfp91 and Cntf has been observed in human and mouse. |
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文献和实验deposit: NoPrice_code: AAvailability in cell line catalogues: ATCC CCL 61; IZSBS BS CL15; DSMZ ACC 110;Bibliographic references:J Exp Med 1958;108:945 Proc Nat Acad Sci USA 1968;60:1275CHO-K1 (hamster, Chinese, ovary)Morphology: epithelial-likeSpecies
后置-20℃保存 20×SSC 10×PBS （DEPC处理的）含50%甲酰胺的杂交液（HS）--- 1ml分装后置-20℃保存 10×阻断液（10×BS） Buffer I Buffer II Buffer III 探针（P） 抗Dig-Ap NBT/BCIP 二、操作流程: 1） 脱蜡至水： 二甲苯5min×2 → 90%乙醇5min → DEPC水洗涤5min×2 2） 杂交前处理： 1×PBS洗涤5min → 蛋白酶K（终浓度20ug/ml in PBS）37℃
in Renal Cancer (p 73-96) Rosamonde E. Banks, Peter J. Selby Chapter 6: Heat Shock Protein 27 in Cancer (p 97-109) Dr. Cecilia Sarto, Dr. Fulvio Magni, BS. Cristina Valsecchi, Prof. Dr. Paolo Mocarelli Chapter 7: Proteomic Approaches for Biomarker
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