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| 规格： | 10ug | 产品价格： | ￥100.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ￥260.0 |
| 规格： | 500ug | 产品价格： | ￥1040.0 |
| 产品编号 | bs-48067P |
| 英文名称 | Recombinant human LR3 IGF1 protein (Active, CHO) |
| 中文名称 | 重组人LR3胰岛素样生长因子1 |
| 英文别名 | Insulin-Like Growth Factor I; IGF-I; Mechano Growth Factor; MGF; Somatomedin-C; IGF1; IBP1; |
| 性状 | Lyophilized |
| 纯化方法 | AC |
| 理论分子量 | 9 |
| 储存液 | Lyophilized from a 0.22μm-filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH7.4 |
| 研究领域 | Cancer > Growth factors > Insulin and insulin-like Developmental Biology > Post embryonic development > Aging Developmental Biology > Post embryonic development > Growth hormones Kits/ Lysates/ Other > Kits > ELISA Kits > ELISA Kits > Growth factors and hormones ELISA kits Signal Transduction > Growth Factors/Hormones > Insulin / Insulin-like |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the insulin family. |
| 功能 | The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 36 months at -20℃ to -80℃ in lyophilized state. 6 months at -20℃ to -80℃ under sterile conditions after reconstitution. 7-10 days at 2℃ to 8℃ under sterile conditions after reconstitution. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009] |
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文献和实验deposit: NoPrice_code: AAvailability in cell line catalogues: ATCC CCL 61; IZSBS BS CL15; DSMZ ACC 110;Bibliographic references:J Exp Med 1958;108:945 Proc Nat Acad Sci USA 1968;60:1275CHO-K1 (hamster, Chinese, ovary)Morphology: epithelial-likeSpecies
后置-20℃保存 20×SSC 10×PBS （DEPC处理的）含50%甲酰胺的杂交液（HS）--- 1ml分装后置-20℃保存 10×阻断液（10×BS） Buffer I Buffer II Buffer III 探针（P） 抗Dig-Ap NBT/BCIP 二、操作流程: 1） 脱蜡至水： 二甲苯5min×2 → 90%乙醇5min → DEPC水洗涤5min×2 2） 杂交前处理： 1×PBS洗涤5min → 蛋白酶K（终浓度20ug/ml in PBS）37℃
in Renal Cancer (p 73-96) Rosamonde E. Banks, Peter J. Selby Chapter 6: Heat Shock Protein 27 in Cancer (p 97-109) Dr. Cecilia Sarto, Dr. Fulvio Magni, BS. Cristina Valsecchi, Prof. Dr. Paolo Mocarelli Chapter 7: Proteomic Approaches for Biomarker
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