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| 规格： | 10ug | 产品价格： | ￥800.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ￥2400.0 |
| 规格： | 500ug | 产品价格： | ￥9600.0 |
| 产品编号 | bs-48056P |
| 英文名称 | Recombinant human IL-18 protein (Active, E.coli) |
| 中文名称 | 重组人白细胞介素18 |
| 英文别名 | Interleukin-18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma;GIF; IL-1g; IL1F4; MGC12320 |
| 性状 | Lyophilized |
| 纯化方法 | AC |
| 理论分子量 | 18.2 |
| 储存液 | Lyophilized from 0.22μm-filtered solution in PBS, 5% mannital, 0.01% Tween 80, pH7.4 |
| 研究领域 | Cancer > Cancer Metabolism > Response to hypoxia Cancer > Invasion/microenvironment > Angiogenesis > Angiogenic growth factors Cancer > Tumor immunology > Cytokines > Interleukins Immunology > Innate Immunity > Cytokines > Interleukins Metabolism > Pathways and Processes > Metabolism processes > Hypoxia |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the IL-1 family. |
| 功能 | Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 36 months at -20℃ to -80℃ in lyophilized state. 6 months at -20℃ to -80℃ under sterile conditions after reconstitution. 7-10 days at 2℃ to 8℃ under sterile conditions after reconstitution. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | IL18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. |
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文献和实验deposit: NoPrice_code: AAvailability in cell line catalogues: ATCC CCL 61; IZSBS BS CL15; DSMZ ACC 110;Bibliographic references:J Exp Med 1958;108:945 Proc Nat Acad Sci USA 1968;60:1275CHO-K1 (hamster, Chinese, ovary)Morphology: epithelial-likeSpecies
后置-20℃保存 20×SSC 10×PBS （DEPC处理的）含50%甲酰胺的杂交液（HS）--- 1ml分装后置-20℃保存 10×阻断液（10×BS） Buffer I Buffer II Buffer III 探针（P） 抗Dig-Ap NBT/BCIP 二、操作流程: 1） 脱蜡至水： 二甲苯5min×2 → 90%乙醇5min → DEPC水洗涤5min×2 2） 杂交前处理： 1×PBS洗涤5min → 蛋白酶K（终浓度20ug/ml in PBS）37℃
in Renal Cancer (p 73-96) Rosamonde E. Banks, Peter J. Selby Chapter 6: Heat Shock Protein 27 in Cancer (p 97-109) Dr. Cecilia Sarto, Dr. Fulvio Magni, BS. Cristina Valsecchi, Prof. Dr. Paolo Mocarelli Chapter 7: Proteomic Approaches for Biomarker
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