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500ug
| 产品编号 | bs-13355P |
| 英文名称 | MBOAT4/Ghrelin O acyltransferase Antibody Blocking Peptide |
| 中文名称 | 脑肠肽O酰基转移酶/Ghrelin O acyltransferase封闭多肽 |
| 英文别名 | FKSG89; ghrelin o transferas; Ghrelin O-acyltransferase; GOAT; MBOA4_HUMAN; Mboat4; Membrane-bound O-acyltransferase domain-containing protein 4; O-acyltransferase (membrane bound) domain containing 4; O-acyltransferase domain-containing protein 4; OACT4. |
| 纯化方法 | HPLC |
| 研究领域 | Metabolism > Types of disease > Diabetes Metabolism > Types of disease > Obesity |
| 亚细胞定位 | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| 组织特异性 | Expressed predominantly in stomach with moderate levels in pancreas and relatively low levels in most other tissues. |
| 相似性 | Belongs to the membrane-bound acyltransferase family. |
| 功能 | Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid. |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | MBOAT4 (membrane-bound O-acyltransferase domain-containing protein 4, Ghrelin O-acyltransferase) is a 435 amino acid, multi-pass membrane protein that belongs to the membrane-bound acyltransferase family. MBOAT4 functions as an enzyme that attaches an octanoate fatty acid to serine-3 of ghrelin. Ghrelin is a very small, appetite-stimulating hormone secreted by the food-deprived stomach. MBOAT4 can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid. MBOAT4 expression, consistent with its function, is mainly in the stomach and intestines. Due to its primary function, MBOAT4 is a candidate for obesity and appetite suppression studies. |
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文献和实验Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
. Fack, F., Deroo, S., Kreis, S., and Muller, C.P. 2000. Heteroduplex mobility assay (HMA) pre‐screening: An improved strategy for the rapid identification of inserts selected from phage‐displayed peptide
0.3% hydrogen peroxide (prepare immediately before use) Blocking buffer (see recipe ) HCV‐specific primary antibody (Ab), e.g
Phage‐Based Expression Cloning to Identify Interacting Proteins
, W., Sellers, W.R., DeCaprio, J.A., Ajchenbaum, F., Fuchs, C.S., Chittenden, T., Li, Y., Farnham, P.J., Blanar, M.A., Livingston, D.M., and Flemington, E.K. 1992. Expression cloning of a cDNA encoding a retinoblastoma‐binding protein with E2F
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